Hierarchical graphical model reveals HFR1 bridging circadian rhythm and flower development in Arabidopsis thaliana

To study the nature of the system-level cell, it is necessary to go beyond the individual regulators and target genes to study the regulatory network between transcription factors (TF). However, it is difficult to immediately dissect TF-mediated gene genome-wide regulatory network (GRN) by experiment. Here, we propose a hierarchical graphical models to estimate activity of TF mRNA expression by building complexes with cofactor TF protein and downstream regulatory networks TF concluded simultaneously.

Then we apply our model to the development of interest and the process of circadian rhythms in Arabidopsis thaliana. The calculations show that a specific sequence family bHLH TF HFR1 recruiting chromatin regulator HAC1 for flower development master regulator TF AG and AG by further activating the expression of histone acetylation. Both independent data and experimental results supported this invention. We also found the flower tissue specific H3K27ac CHIP-Seq peaks in the AG gene and motif HFR1 body in the middle of this H3K27ac peak. In addition, we verified that HFR1 physically interact with HAC1 by yeast two-hybrid experiments.

HFR1 relationship-HAC1 triplet-AG may imply that the development of flowers and circadian rhythms are bridged by epigenetic regulation and enrich the classical ABC model in the development of flowers. In addition, our network TF activity may serve as a general method to explain the molecular mechanisms in more complex biological regulatory processes.
In the male germline plant, transposable elements (TES) were reactivated in the accompanying vegetative nucleus, which results from their siRNA production and movement of the shuttle is to strengthen TE siRNAs silencing in sperm.

However, the mechanism by which siRNA movement arranged the remains unexplored. Here we show that ARID1, a transcription factor that is constitutively expressed in vegetative nucleus but dynamic accumulated in generative cells (progenitor sperm) to promote the second pollen mitosis, mediates movement heterochromatic silencing siRNA to strengthen in the male germline.

 Hierarchical graphical model reveals HFR1 bridging circadian rhythm and flower development in Arabidopsis thaliana
Hierarchical graphical model reveals HFR1 bridging circadian rhythm and flower development in Arabidopsis thaliana

direct inhibition of photosynthesis by Cd dominate inhibition caused by micronutrient deficiencies in Cd / Zn hiperakumulator Arabidopsis halleri

This work revealed by imaging in vivo measurements in Cd / Zn hiperakumulator Arabidopsis halleri, how much stress Cd affects macronutrients (Ca, K) and micronutrients (Fe, Zn) distribution in the leaves. We directly correlate these changes with the biophysics of the light reactions of photosynthesis.

Plants were grown for 2 months at 10 pM Zn (= control), and supplemented with 10, 15, 50 or 75 pM Cd. Direct imaging of transient OJIP revealed that the bundle sheath cells more sensitive to the toxicity of Cd from mesophyll cells away from the vein. progressive inhibition of photosystem (PS) II reaction center and the quantum yield reduction of electron transport between QA and QB and then to the PSI acceptor observed. This correlates with a decrease in the dynamics of QA re-oxidation and decrease the operational efficiency of PSII.

Analysis by X-ray fluorescence device benchtop micro showed that most of the Cd accumulates in the blood vessels, and bounded Fe and Zn distribution of blood vessels, especially in the 75 pM Cd, while K concentration increased throughout the leaves. calcium distribution seems unaffected by Cd, but Cd excess trichomes inhibited the formation and thus reduced concentration of total Ca in the leaves. The results show differential tissue sensitivity to Cd, as evidenced by the inhibition of photosynthesis heterogeneous.

Genomic DNA - Plant Normal Tissue: Arabidopsis

D1634310-5 5 ug
EUR 329

cDNA - Plant Normal Tissue: cDNA - Plant: Corn

C1634330 40 reactions
EUR 741

cDNA - Plant Normal Tissue: cDNA - Plant: Rice

C1634360 40 reactions
EUR 741

cDNA - Plant Normal Tissue: cDNA - Plant: Wheat

C1634390 40 reactions
EUR 741

cDNA - Plant Normal Tissue: cDNA - Plant: Orange

C1634340 40 reactions
EUR 741

cDNA - Plant Normal Tissue: cDNA - Plant: Potato

C1634350 40 reactions
EUR 741

cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean

C1634370 40 reactions
EUR 741

Supreme Plant Tissue Culture Grade Agar

PCTA100 500g
EUR 144
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

Supreme Plant Tissue Culture Grade Agar

PCTA500 1000g
EUR 180
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

Plant: Arabidopsis Lysate

21-395 0.1 mg
EUR 342.6
Description: Plant plant-arabidopsis matter lysate was prepared by homogenization using a proprietary technique. The matter was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The plant plant-arabidopsis matter total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the plant-arabidopsis matter on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The plant-arabidopsis matter is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Arabidopsis thaliana Plant Lysate

GWB-ECFF87 0.15 mg Ask for price

Plant-Derived Arabidopsis Lysate

IPLTARATL100UG each
EUR 541
Description: Plant-Derived Arabidopsis Lysate

Plant Preservative Mixture

PCT01 30 ml
EUR 108
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Plant Preservative Mixture

PCT02 100 ml
EUR 898.8
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Plant Preservative Mixture

PCT03 250 ml
EUR 372
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Plant Preservative Mixture

PCT04 500 ml
EUR 540
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Plant Preservative Mixture PPM

PCT05 1000 ml
EUR 958.8
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Total RNA - Plant Normal Tissue: Corn

R1634330 100 ug
EUR 775

Total RNA - Plant Normal Tissue: Rice

R1634360 100 ug
EUR 775

Total RNA - Plant Normal Tissue: Wheat

R1634390 100 ug
EUR 775

Arabidopsis cDNA

PLD-1021 30 reactions
EUR 498

Genomic DNA - Plant Normal Tissue: Corn

D1634330 10 ug
EUR 244

Genomic DNA - Plant Normal Tissue: Rice

D1634360 10 ug
EUR 244

Total RNA - Plant Normal Tissue: Orange

R1634340 100 ug
EUR 775

Total RNA - Plant Normal Tissue: Potato

R1634350 100 ug
EUR 775

Genomic DNA - Plant Normal Tissue: Wheat

D1634390 10 ug
EUR 244

Genomic DNA - Plant Normal Tissue: Orange

D1634340 10 ug
EUR 244

Genomic DNA - Plant Normal Tissue: Potato

D1634350 10 ug
EUR 244

Total RNA - Plant Normal Tissue: Soy bean

R1634370 100 ug
EUR 775

Genomic DNA - Plant Normal Tissue: Soy bean

D1634370 10 ug
EUR 244

Arabidopsis Thaliana Whole Tissue Lysate

PABL-1300 50 ug
EUR 196.8

cDNA - Rat Normal Tissue: Placenta

C1434200 40 reactions
EUR 636

cDNA - Mouse Normal Tissue: Placenta

C1334200 40 reactions
EUR 636

cDNA - Human Adult Normal Tissue: Placenta

C1234200 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Lung

C1734152 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Lung

C1434152 40 reactions
EUR 424

cDNA - Monkey (Rhesus) Normal Tissue: Placenta

C1534200 40 reactions
EUR 481

cDNA - Dog Normal Tissue: Brain

C1734035 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Cecum

C1734089 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Heart

C1734122 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Liver

C1734149 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Brain

C1434035 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Colon

C1434090 40 reactions
EUR 636

cDNA - Rat Normal Tissue: Heart

C1434122 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Liver

C1434149 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Kidney

C1734142 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Rectum

C1734206 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Testis

C1734260 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Lung

C1334152 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Kidney

C1434142 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Rectum

C1434206 40 reactions
EUR 636

cDNA - Rat Normal Tissue: Spleen

C1434246 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Testis

C1434260 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Bladder

C1734010 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Trachea

C1734160 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Stomach

C1734248 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Brain

C1334035 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Colon

C1334090 40 reactions
EUR 636

cDNA - Mouse Normal Tissue: Heart

C1334122 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Liver

C1334149 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Adipose

C1434003 40 reactions
EUR 636

cDNA - Rat Normal Tissue: Bladder

C1434010 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Stomach

C1434248 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Pancreas

C1734188 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Kidney

C1334142 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Rectum

C1334206 40 reactions
EUR 636

cDNA - Mouse Normal Tissue: Spleen

C1334246 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Testis

C1334260 40 reactions
EUR 424

cDNA - Monkey (Cynomolgus) Normal Tissue: Placenta

C1534200-Cy 40 reactions
EUR 481

cDNA - Dog Normal Tissue: Esophagus

C1734106 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Adipose

C1334003 40 reactions
EUR 636

cDNA - Mouse Normal Tissue: Bladder

C1334010 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Stomach

C1334248 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Lung

C1234152 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Skin

C1234218 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Brain

C1234035 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Colon

C1234090 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Heart

C1234122 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Liver

C1234149 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Ovary

C1234183 40 reactions
EUR 1154

cDNA - Human Adult Normal Tissue: Theca

C1234261-10 10 reactions
EUR 237

cDNA - Monkey (Rhesus) Normal Tissue: Lung

C1534152 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Skin

C1534218 40 reactions
EUR 481

cDNA - Human Adult Normal Tissue: Breast

C1234086 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Kidney

C1234142 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Rectum

C1234206 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Spleen

C1234246 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Testis

C1234260 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Throat

C1234263-10 10 reactions
EUR 237

cDNA - Human Adult Normal Tissue: Thymus

C1234264 40 reactions
EUR 1154

cDNA - Human Adult Normal Tissue: Tongue

C1234267-10 10 reactions
EUR 237

cDNA - Human Adult Normal Tissue: Tonsil

C1234268-10 10 reactions
EUR 237

cDNA - Human Adult Normal Tissue: Uterus

C1234274 40 reactions
EUR 424

cDNA - Monkey (Rhesus) Normal Tissue: Brain

C1534035 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Cecum

C1534089 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Colon

C1534090 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Heart

C1534122 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Liver

C1534149 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Ovary

C1534183 40 reactions
EUR 1311

cDNA - Monkey (Rhesus) Normal Tissue: Penis

C1534194 40 reactions
EUR 723

cDNA - Dog Normal Tissue: Skeletal Muscle

C1734171 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Small Intestine

C1734226 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Skeletal Muscle

C1434171 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 636

cDNA - Human Adult Normal Tissue: Adipose

C1234003-10 10 reactions
EUR 237

cDNA - Human Adult Normal Tissue: Adrenal

C1234004 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Bladder

C1234010 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Trachea

C1234160-10 10 reactions
EUR 237

cDNA - Human Adult Normal Tissue: Stomach

C1234248 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Thyroid

C1234265 40 reactions
EUR 424

cDNA - Monkey (Rhesus) Normal Tissue: Kidney

C1534142 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Rectum

C1534206 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Spleen

C1534246 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Testis

C1534260 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Throat

C1534263 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Thymus

C1534264 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Tongue

C1534267 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Tonsil

C1534268 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Ureter

C1534273 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Uterus

C1534274 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Vagina

C1534283 40 reactions
EUR 723

cDNA - Human Adult Normal Tissue: Pancreas

C1234188 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Prostate

C1234201 40 reactions
EUR 424

cDNA - Monkey (Rhesus) Normal Tissue: Adipose

C1534003 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Adrenal

C1534004 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Bladder

C1534010 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Trachea

C1534160 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Parotid

C1534190 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Stomach

C1534248 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Thyroid

C1534265 40 reactions
EUR 723

cDNA - Mouse Normal Tissue: Skeletal Muscle

C1334171 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Small Intestine

C1334226 40 reactions
EUR 636

cDNA - Human Adult Normal Tissue: Esophagus

C1234106 40 reactions
EUR 424

cDNA - Monkey (Rhesus) Normal Tissue: Appendix

C1534006 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Lung

C1534152-Cy 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Pancreas

C1534188 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Prostate

C1534201 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Skin

C1534218-Cy 40 reactions
EUR 481

cDNA - Monkey (Cynomolgus) Normal Tissue: Brain

C1534035-Cy 40 reactions
EUR 481

cDNA - Monkey (Cynomolgus) Normal Tissue: Cecum

C1534089-Cy 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Colon

C1534090-Cy 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Esophagus

C1534106 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Heart

C1534122-Cy 40 reactions
EUR 481

cDNA - Monkey (Cynomolgus) Normal Tissue: Liver

C1534149-Cy 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Diaphragm

C1534169 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Ovary

C1534183-Cy 40 reactions
EUR 1311

cDNA - Monkey (Cynomolgus) Normal Tissue: Penis

C1534194-Cy 40 reactions
EUR 723

Monkey (Cynomolgus) cDNA Normal Tissue: Liver

MC34-149 10 rxn
EUR 498

cDNA - Monkey (Rhesus) Normal Tissue: Epididymis

C1534105 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Kidney

C1534142-Cy 40 reactions
EUR 481

cDNA - Monkey (Cynomolgus) Normal Tissue: Rectum

C1534206-Cy 40 reactions
EUR 481

cDNA - Monkey (Cynomolgus) Normal Tissue: Spleen

C1534246-Cy 40 reactions
EUR 481

cDNA - Monkey (Cynomolgus) Normal Tissue: Testis

C1534260-Cy 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Throat

C1534263-Cy 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Thymus

C1534264-Cy 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Tongue

C1534267-Cy 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Tonsil

C1534268-Cy 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Ureter

C1534273-Cy 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Uterus

C1534274-Cy 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Vagina

C1534283-Cy 40 reactions
EUR 723

Monkey (Cynomolgus) cDNA Normal Tissue: Spleen

MC34-246 10 rxn
EUR 498

cDNA - Monkey (Cynomolgus) Normal Tissue: Adipose

C1534003-Cy 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Adrenal

C1534004-Cy 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Bladder

C1534010-Cy 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Gallbladder

C1534118 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Trachea

C1534160-Cy 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Parathyroid

C1534189 40 reactions
EUR 1475

cDNA - Monkey (Cynomolgus) Normal Tissue: Parotid

C1534190-Cy 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Stomach

C1534248-Cy 40 reactions
EUR 481

Part of this may result in the selective disruption of homeostasis of leaf nutrients. Photosynthesis better performance away from the veins compared to the bundle sheath cells, however, showed that direct inhibition of photosynthesis by Cd dominate inhibition caused by micronutrient deficiencies.