Deregulated High Affinity Copper Transport Alters Iron Homeostasis in Arabidopsis
This work describes the effects on iron homeostasis when copper transport was deregulated in Arabidopsis thaliana by overexpressing the high affinity copper transporter COPT1 and COPT3 (COPTOE). A genome-wide analysis carried out on the plant COPT1OE, highlighting that iron homeostasis affected the expression of genes under both copper deficiency and excess. Among the genes that change is their coding machine iron absorption and their transcriptional regulators. Furthermore, COPTOE seeds contained less iron and more sensitive than the control of iron deficiency.
Deregulation of copper (I) uptake inhibiting the transcriptional activation of Ib subgroup of basic helix-loop-helix (bHLH-Ib) factor under copper deficiency. In fact, excess copper inhibits the expression of master regulators FIT but activated bHLH-lb expression in plants COPTOE, in either case leading to a lack of response to an adequate iron absorption. Increase in media such as copper, iron (III) has been accumulated in the roots, and the ratio of iron (III) / iron (II) increases in crop COPTOE.
Thus, iron (III) COPTOE root overloading in iron deficiency inhibit the response of local, aims to metal uptake from the soil, leading to a general low iron content in seeds COPTOE. These results emphasize the importance of proper spatiotemporal copper absorption of iron homeostasis under non-optimal supply of copper. An understanding of the role of copper absorption in iron metabolism can be applied to improve plant resistance to iron deficiency.
Deregulated High Affinity Copper Transport Alters Iron Homeostasis in Arabidopsis
A single-population AtMATE GWAS identified the expression level of polymorphism is caused by the promoter variant associated with variations in aluminum tolerance in Arabidopsis local population
organic acid (OA) is released from the roots in response to aluminum (Al), conferring Al tolerance in plants are regulated by OA transporters such as ALMT (Al-activated malate transporter) and multidrug and toxic compound extrusion (MATE). We have previously reported that the expression level of polymorphism (ELP) from AtALMT1 strongly associated with variations in Al tolerance between natural Arabidopsis accessions.
However, although AtMATE also states follow Al exposure and contributed to Al tolerance, whether AtMATE contributes to the variation of Al tolerance and molecular mechanisms of the ELP is still unclear. Here, we dissect the natural variations in expression levels AtMATE in response to Al at the root using diverse natural accessions of Arabidopsis. Phylogenetic analysis showed that more than half of the population show accessions belonging to Central Asia (CA) real AtMATE low expression levels, while the majority of European populations show a high level of expression. Accession of the population AtMATE CA with low expression also showed significantly weakened Al tolerance.
A genome-wide association study of single-resident (GWAS) of AtMATE expression in CA identifying populations at AtMATE retrotransposon insertion of the promoter region of genes associated with a low level of expression. This can affect the transcriptional regulation of AtMATE by interfering with the effects of cis-regulatory elements located upstream of the insertion site, which include AtSTOP1 (sensitive to proton rhizotoxicity 1) transcription factor-binding sites revealed by the analysis of chromatin immunoprecipitation-qPCR.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Mouse Normal Tissue: Small Intestine
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Rat Normal Tissue: Skeletal Muscle
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Rat Normal Tissue: Small Intestine
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
FFPE Genomic DNA - Human Adult Normal Tissue: Colon
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
FFPE Genomic DNA - Human Adult Normal Tissue: Liver
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
FFPE Genomic DNA - Human Adult Normal Tissue: Lung
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
FFPE Genomic DNA - Human Adult Normal Tissue: Pancreas
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Plant plant-arabidopsis matter lysate was prepared by homogenization using a proprietary technique. The matter was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The plant plant-arabidopsis matter total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the plant-arabidopsis matter on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The plant-arabidopsis matter is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Orange
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Potato
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Rice
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Wheat
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Adipose, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Adrenal, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Brain, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Breast, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Colon, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Esophagus, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Heart, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Kidney, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Liver, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Lung, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Pancreas, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Placenta, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Rectum, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Ileum, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Spleen, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Stomach, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Testis, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Uterus, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
FFPE and Frozen Matching Pair Genomic DNA: Human Adult Normal Tissue: Liver
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
96-well Plant Genomic DNA Extraction Kit (1 plates)
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Heart: Pericardium, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Skeletal Muscle, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Small Intestine, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Uterus: Cervix, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
EZ-500 Spin Column Plant Genomic DNA Maxi-Preps Kit
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Heart: Ventricle, left, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Human Adult Normal Tissue: Peripheral Blood Leukocyte, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA - Human Diabetic Tissue: Brain, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA - Human Diabetic Tissue: Colon, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA - Human Diabetic Tissue: Esophagus, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA - Human Diabetic Tissue: Heart, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA - Human Diabetic Tissue: Kidney, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA - Human Diabetic Tissue: Liver, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA - Human Diabetic Tissue: Lung, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA - Human Diabetic Tissue: Pancreas, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA - Human Diabetic Tissue: Skin, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA - Human Diabetic Tissue: Spleen, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
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Furthermore, GWAS done without accession express low levels of AtMATE, excluding the effect AtMATE promoter polymorphisms, identified several potential candidate genes associated with AtMATE expression.
