The Role of Flavonol Synthesized by Nucleus FLS1 in Arabidopsis Resistance to Pb Stress

August 20, 2020
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The lead (Pb) is an important pollutant of concern to the whole world due to extensive pollution sources and effects of which are highly toxic. Flavanols can increase plant resistance to abiotic stresses, is also responsible for the effect of reducing under pressure Pb. The relationship between stress and flavonols Pb, still, the knowledge about the mechanism of function of flavonols are very limited. Pb affect the metabolism of energy, thereby inhibiting plant growth and development.

Flavonol accumulation is controlled by FLS1 (flavonol synthase) could alleviate the toxic effects. Importantly, nes (Mutants of NES allows FLS1 enter expressions nucleus) indicate the status of better growth and oxidative damage lighter than the NES (N-terminal core exception signal peptide prevents FLS1 entering the expression core), which indicates that the core-flavonol synthesized by FLS1 core plays a key role in plant resistance to stress Pb.

Although FLS1 signal detected in the cell membrane, cytoplasm and nucleus-flavonol membrane, cytoplasm and nucleus-flavonol-flavonol who do not exercise their functions in the appropriate position. FLS1 core expression intervene in the content and composition of total flavonols. The results also reveal that the core-flavonol may regulate metabolism Ascorbic to reduce damage to the chloroplast, thereby maintaining photophosphorylation path.

Our findings provide new insights into the molecular basis of tolerance Pb and mechanisms of plant responses.
Quorum sensing (QS) is a phenomenon recognizes that is very important to regulate the behavior of related bacterial population. However, special effects direct from the molecular biology of host QS largely understudied. In this work, we show that the DSF QS molecule (cis-11-methyl-dodecenoic acid) produced by Xanthomonas campestris pv. can suppress immune campestris pathogen-associated molecular patterns triggered (PTI) in Arabidopsis thaliana, mediated by flagellin-induced activation of receptor FLS2 flagellin.

DSF-mediated attenuation of innate immunity results from nanoclusters changes FLS2 and endocytic internalization of plasma membrane FLS2. DSF changed the lipid profile of Arabidopsis, with a particular increase in the species phytosterols, which disrupted the general endocytosis mediated by clathrin and FLS2 nano-clustering on the plasma membrane. DSF effect on the dynamics of the receptor and the host immune response can be completely reversed with the removal of sterols.

The Role of Flavonol Synthesized by Nucleus FLS1 in Arabidopsis Resistance to Pb Stress
The Role of Flavonol Synthesized by Nucleus FLS1 in Arabidopsis Resistance to Pb Stress

cold acclimation has a different effect on the leaf level of the export structure and carbon vascular bundles in the natural Arabidopsis accessions originating from southern and northern Europe

Acclimation to low but non-freezing temperatures is an important ecological process for Arabidopsis thaliana, but also for many other plant species from temperate regions. consisting of cold acclimation and affect various physiological and molecular processes and supply of sugar from the network maintenance sunk by a network of photosynthetically active source is essential for the survival of plants. Here, changes in vascular structure bundles (VB) on leaf petioles are analyzed together with the level of exudate sucrose before and after cold acclimation.

Six natural Arabidopsis accessions coming from the south and the north of Europe compared. the efficiency of photosynthesis, ie the maximum and effective quantum results photosystem II, revealed a significant effect of environmental conditions. Just to the North accession is a very significant negative correlation was observed between the level of sucrose leaf exudation, xylem, and the cross-sectional area of ​​the petiole.

Genomic DNA - Plant Normal Tissue: Arabidopsis
D1634310 10 ug
EUR 629

Genomic DNA - Plant Normal Tissue: Arabidopsis
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Tissue, Genomic DNA, Plant Normal, Plant, Arabidopsis, BioGenomics
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Tissue, Genomic DNA, Plant Normal, Plant, Arabidopsis, BioGenomics
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Tissue, Total RNA, Plant Normal, Plant, Orange, BioGenomics
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

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In addition, only for North accession is a significant increase of VB and leaf petiole cross-sectional area was observed during cold acclimation.

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