Description: T helper cells (Th) play a vital role in the adaptive immune system by suppressing or regulating immune responses. After Th actived, it will differentiate into T-helper 1 (Th1) or T-helper 2 (Th2) effector cells. These two subsets are characterized by their distinct cytokine expression pattern and the immune function they mediate. Th1 and Th2 cells each produce a specialized combination of cytokines that depends on type of pathogen that the helper T cell has recognized—some cytokines are more effective than others in the process of eliminating certain invaders. In general, TH1 cytokines play the role of immune killing, while Th2 cytokines play the role of immune protection. Th1/Th2 paradigm provides rationale for developing new vaccines against infectious diseases and novel strategies for the therapy of allergic and autoimmune disorders.

Description: The PDE4D cell-based activity assay is designed for screening inhibitors of PDE4D7 in cultured cells. The assay is based on transfecting cells with the CRE luciferase reporter. _x000D_CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter. However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity.

Description: Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. PDE4B has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP. Inhibition of PDE4B activity by its inhibitors leads to an elevated intracellular level of cAMP. The PDE4B gene encodes at least 4 different isoforms, and has been linked to inflammation in monocytes by its regulation of the toll-receptor pathway. It is also highly expressed in the central nervous system, and has been targeted as a potential treatment for autism, depression, schizophrenia, and other conditions._x000D_The PDE4B cell-based activity assay kit is designed for screening inhibitors of PDE4B1 in cultured cells. The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter. However, when cells are co-transfected with PDE4B1 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4B inhibitor to inhibit PDE4B1 activity, cAMP level is restored, resulting in higher luciferase activity._x000D_The kit includes CRE luciferase reporter (premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency), PDE4B1 expression vector, and forskolin.

Description: The human IDO1 Cell-Based Assay Kit is designed to monitor the activity of exogenously expressed human IDO1 in cultured cells.

Description: Streptavidin-HRP is useful in select BPS Bioscience assay kits.

Description: Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide.As the virus spreads globally, the continuous emergence of new mutant strains escalated the challenge on humans.To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure IgG antibodies against the mutants is in urgent need.

Description: Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide. Omicron is a variant of SARS-CoV-2 first detected in November 2021 and is causing the ongoing COVID-19 pandemic. The Omicron subvariants BA.1 and BA.2 of SARS-CoV-2 have dominated the COVID-19 pandemic in early 2022. Its recent descendants BA.2.12.1 and BA.4/5 have surged dramatically to become dominant in the United States and South Africa, respectively. BA.2.12.1 and BA.4/BA.5 exhibit comparable ACE2-binding affinities to BA.2. Importantly, BA.2.12.1 and BA.4/BA.5 display stronger neutralization evasion than BA.2 against the plasma from 3-dose vaccination and, most strikingly, from post-vaccination BA.1 infections.

Description:

Product Overview

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The Multiplex Human Cytokine – Screen (16-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Multiple analyte measurements are achieved by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well of a 96-well plate. Our high-quality reagents help ensure the accuracy and precision of your results.

Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.25 hours. The Multiplex Human Cytokine – Screen (16-plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample.

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Assay Ranges

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Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
IFNγ	Sandwich	8000 -10.97 (pg/mL)	5600 (pg/mL)	14.3 (pg/mL)	4.95 (pg/mL)
IL-1α	Sandwich	4000 -5.49 (pg/mL)	2800 (pg/mL)	7.1 (pg/mL)	5.43 (pg/mL)
IL-1β	Sandwich	12226 -16.77 (pg/mL)	8558 (pg/mL)	21.8 (pg/mL)	12.40 (pg/mL)
IL-2	Sandwich	5213 – 7.15 (pg/mL)	3649 (pg/mL)	9.3 (pg/mL)	4.12 (pg/mL)
IL-4	Sandwich	1676 – 2.30 (pg/mL)	1173 (pg/mL)	3.0 (pg/mL)	1.79 (pg/mL)
IL-5	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	2.90 (pg/mL)
IL-6	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	3.47 (pg/mL)
IL-8	Sandwich	2000 – 2.74 (pg/mL)	1400 (pg/mL)	3.6 (pg/mL)	1.37 (pg/mL)
IL-10	Sandwich	8039 – 11.03 (pg/mL)	5627 (pg/mL)	14.3 (pg/mL)	10.99 (pg/mL)
IL-12p70	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	3.20 (pg/mL)
IL-13	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	4.12 (pg/mL)
IL-15	Sandwich	10733 – 14.72 (pg/mL)	7513 (pg/mL)	19.1 (pg/mL)	6.87 (pg/mL)
IL-17A	Sandwich	16772 – 23.01 (pg/mL)	11740 (pg/mL)	29.9 (pg/mL)	22.57 (pg/mL)
IL-23	Sandwich	41700 – 57.20 (pg/mL)	21000 (pg/mL)	120 (pg/mL)	48.65 (pg/mL)
TNFα	Sandwich	6500 – 8.92 (pg/mL)	4522 (pg/mL)	11.5 (pg/mL)	7.74 (pg/mL)
TNFβ	Sandwich	4000 – 5.49 (pg/mL)	2800 (pg/mL)	7.1 (pg/mL)	5.29 (pg/mL)

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Kit Components

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Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.

_x000D_

Reagents Include:

_x000D_

Calibrator: Lyophilized, recombinant antigens in a buffered protein base;Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives ;Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide ;Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer ;Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives ;Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;Plate Seals (3): Adhesive strips

Description:

Product Overview

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The Multiplex Mouse Cytokine – Screen (15-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 15 biomarkers or analytes. Using just 25 µl of sample per well, up to 80 samples can be assayed for all 15 markers in the panel within 5.5 hours.

The Mouse Cytokine – Screen (15-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 15 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

_x000D_

Assay Ranges

_x000D__x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_

Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
GMCSF	Sandwich	560 - 0.77 (pg/mL)	392 (pg/mL)	1 (pg/mL)	0.38 (pg/mL)
IFNγ	Sandwich	516 - 0.71 (pg/mL)	361 (pg/mL)	0.94 (pg/mL)	0.35 (pg/mL)
IL-1α	Sandwich	610 - 0.84 (pg/mL)	427 (pg/mL)	1.1 (pg/mL)	0.42 (pg/mL)
IL-1β	Sandwich	2500 - 3.43 (pg/mL)	1750 (pg/mL)	4.5 (pg/mL)	1.71 (pg/mL)
IL-2	Sandwich	360 - 0.49 (pg/mL)	252 (pg/mL)	0.6 (pg/mL)	0.25 (pg/mL)
IL-3	Sandwich	60 - 0.08 (pg/mL)	42 (pg/mL)	0.1 (pg/mL)	0.04 (pg/mL)
IL-4	Sandwich	400 - 0.55 (pg/mL)	280 (pg/mL)	0.7 (pg/mL)	0.27 (pg/mL)
IL-5	Sandwich	546 - 0.75 (pg/mL)	382 (pg/mL)	0.96 (pg/mL)	0.37 (pg/mL)
IL-6	Sandwich	504 - 0.69 (pg/mL)	353 (pg/mL)	0.91 (pg/mL)	0.35 (pg/mL)
IL-10	Sandwich	667 - 0.91 (pg/mL)	467 (pg/mL)	1.33 (pg/mL)	0.46 (pg/mL)
IL-12p70	Sandwich	572 - 0.78 (pg/mL)	400 (pg/mL)	1.01 (pg/mL)	0.39 (pg/mL)
IL-17A	Sandwich	760 - 1.04 (pg/mL)	532 (pg/mL)	1.4 (pg/mL)	0.52 (pg/mL)
MIP-1α	Sandwich	1000 - 1.37 (pg/mL)	700 (pg/mL)	1.8 (pg/mL)	0.69 (pg/mL)
RANTES	Sandwich	1653 - 2.27 (pg/mL)	1157 (pg/mL)	2.92 (pg/mL)	1.13 (pg/mL)
TNFα	Sandwich	429 - 0.59 (pg/mL)	300 (pg/mL)	0.69 (pg/mL)	0.29 (pg/mL)

_x000D_

Kit Components

_x000D_

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format, please contact us for customization.

_x000D_

Reagents Include:

_x000D_

Calibrator: Lyophilized, recombinant antigens in a buffered protein base;Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives ;Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide ;Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer ;Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives ;Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;Plate Seals (3): Adhesive strips

Description:

Product Overview

_x000D_

The Multiplex Porcine Cytokine – High Sensitivity (4-plex) is a fully quantitative, ELISA-based chemiluminescent assay allowing concurrent measurement of cytokines in serum or plasma samples.

A kit contains a 96-well plate, with each well featuring the 4-plex along with a positive control with all the other reagents necessary to perform the test. Our high-quality reagents help ensure the accuracy of your results.

Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 4.83 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.

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Assay Ranges

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Analytes	Calibrator Range	Units	LLD
IL-1β	1550 - 2.13	pg/mL	2.1
IL-6	1800 - 2.47	pg/mL	2.5
IL-8	2100 - 2.88	pg/mL	2.9
TNFα	1600 - 2.19	pg/mL	1.9

_x000D_

Kit Components

_x000D_

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.

_x000D_

Reagents Include:

_x000D_

Calibrator: Lyophilized, recombinant antigens in a buffered protein base;Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives ;Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide ;Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer ;Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with blockers and preservatives ;Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;Plate Seals (3): Adhesive strips

Description:

Product Overview

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The Multiplex Human Cytokine Panel 1 (6-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of six biomarkers or analytes (IL-1β, IL-6, TNFα, IFN-γ, IL-1α, IL-10).

Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing six distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Using only 25 µl of sample per well, up to 80 samples can be assayed for all six markers in the panel in 2.25 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.

_x000D_

Assay Ranges

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Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
IFNγ	Sandwich	8000 -10.97 (pg/mL)	5600 (pg/mL)	14.3 (pg/mL)	4.95 (pg/mL)
IL-1α	Sandwich	4000 -5.49 (pg/mL)	2800 (pg/mL)	7.1 (pg/mL)	5.43 (pg/mL)
IL-1β	Sandwich	12226 -16.77 (pg/mL)	8558 (pg/mL)	21.8 (pg/mL)	12.40 (pg/mL)
IL-6	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	3.47 (pg/mL)
IL-10	Sandwich	8039 – 11.03 (pg/mL)	5627 (pg/mL)	14.3 (pg/mL)	10.99 (pg/mL)
TNFα	Sandwich	6500 – 8.92 (pg/mL)	4522 (pg/mL)	11.5 (pg/mL)	7.74 (pg/mL)

_x000D_

Kit Components

_x000D_

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.

_x000D_

Reagents Include:

_x000D_

Calibrator: Lyophilized, recombinant antigens in a buffered protein base;Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives;Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide;Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer;Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives;Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;Plate Seals (3): Adhesive strips

Description:

Product Overview

_x000D_

The Multiplex Mouse Cytokine Panel 1 (6-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of six biomarkers or analytes (IFN-γ, IL-1α, IL-1β, IL-6, IL-10, TNFα).

Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing six distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Using just 25 µl of sample per well, up to 80 samples can be assayed for all six markers in the panel in 5.5 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.

_x000D_

Assay Ranges

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Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
IFNγ	Sandwich	516 - 0.71 (pg/mL)	361 (pg/mL)	0.94 (pg/mL)	0.35 (pg/mL)
IL-1α	Sandwich	610 - 0.84 (pg/mL)	427 (pg/mL)	1.1 (pg/mL)	0.42 (pg/mL)
IL-1β	Sandwich	2500 - 3.43 (pg/mL)	1750 (pg/mL)	4.5 (pg/mL)	1.71 (pg/mL)
IL-6	Sandwich	504 - 0.69 (pg/mL)	353 (pg/mL)	0.91 (pg/mL)	0.35 (pg/mL)
IL-10	Sandwich	667 - 0.91 (pg/mL)	467 (pg/mL)	1.33 (pg/mL)	0.46 (pg/mL)
TNFα	Sandwich	429 - 0.59 (pg/mL)	300 (pg/mL)	0.69 (pg/mL)	0.29 (pg/mL)

_x000D_

Kit Components

_x000D_

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.

_x000D_

Reagents Include:

_x000D_

Calibrator: Lyophilized, recombinant antigens in a buffered protein base;Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives;Substrate A: Liquid, 3.5 mL/vial ;Substrate B: Liquid, 3.5 mL/vial;Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution;Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;Plate Seals (3): Adhesive strips

Description:

Product Overview

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The Multiplex Mouse Cytokine Panel 2 (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IFNγ, IL-1β, IL-6, TNFα).

Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 5.5 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.

_x000D_

Assay Ranges

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Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
IFNγ	Sandwich	516 - 0.71 (pg/mL)	361 (pg/mL)	0.94 (pg/mL)	0.35 (pg/mL)
IL-1β	Sandwich	2500 - 3.43 (pg/mL)	1750 (pg/mL)	4.5 (pg/mL)	1.71 (pg/mL)
IL-6	Sandwich	504 - 0.69 (pg/mL)	353 (pg/mL)	0.91 (pg/mL)	0.35 (pg/mL)
TNFα	Sandwich	429 - 0.59 (pg/mL)	300 (pg/mL)	0.69 (pg/mL)	0.29 (pg/mL)

_x000D_

Kit Components

_x000D_

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.

_x000D_

Reagents Include:

_x000D_

Calibrator: Lyophilized, recombinant antigens in a buffered protein base;Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives;Substrate A: Liquid, 3.5 mL/vial;Substrate B: Liquid, 3.5 mL/vial;Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution;Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;Plate Seals (3): Adhesive strips

Description:

Product Overview

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The Multiplex ELISA Kit For Human Cytokine Panel 2 (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IL-1α, IL-4, IL-6, TNFα).

Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 2.25 hours. Multiplex ELISA Kit For Human Cytokine Panel 2 (4-Plex) provides scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.

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Assay Ranges

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Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
IL-1α	Sandwich	4000 -5.49 (pg/mL)	2800 (pg/mL)	7.1 (pg/mL)	5.43 (pg/mL)
IL-4	Sandwich	1676 – 2.30 (pg/mL)	1173 (pg/mL)	3.0 (pg/mL)	1.79 (pg/mL)
IL-6	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	3.47 (pg/mL)
TNFα	Sandwich	6500 – 8.92 (pg/mL)	4522 (pg/mL)	11.5 (pg/mL)	7.74 (pg/mL)

_x000D__x000D_

Kit Components

_x000D_

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

_x000D_

Reagents Include:

_x000D_

Calibrator: Lyophilized, recombinant antigens in a buffered protein base;_x000D_Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives;_x000D_Sample Diluent: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives;_x000D_Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;_x000D_Substrate A: Liquid, 3mL/vial of stabilized hydrogen peroxide;Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer;_x000D_Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;Plate Seals (3): Adhesive strips

Description:

Product Overview

_x000D_

The Multiplex ELISA Kit For Mouse Cytokine Panel 3 (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IL-2, IL-4, IL-6, TNFα).

Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 5.5 hours. Multiplex ELISA Kit For Mouse Cytokine Panel 3 (4-Plex) provides scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.

_x000D__x000D_

Assay Ranges

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Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
IL-2	Sandwich	360 - 0.49 (pg/mL)	252 (pg/mL)	0.6 (pg/mL)	0.25 (pg/mL)
IL-4	Sandwich	400 - 0.55 (pg/mL)	280 (pg/mL)	0.7 (pg/mL)	0.27 (pg/mL)
IL-6	Sandwich	504 - 0.69 (pg/mL)	353 (pg/mL)	0.91 (pg/mL)	0.35 (pg/mL)
TNFα	Sandwich	429 - 0.59 (pg/mL)	300 (pg/mL)	0.69 (pg/mL)	0.29 (pg/mL)

_x000D__x000D_

Kit Components

_x000D_

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

_x000D__x000D_

Reagents Include:

_x000D_

Calibrator: Lyophilized, recombinant antigens in a buffered protein base;_x000D_Detection Mix: Lyophilized, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives;_x000D_Sample Diluent: Liquid, 10 mL/vial of a buffered protein solution;_x000D_Substrate A: Liquid, 3.5 mL/vial;_x000D_Substrate B+: Liquid, 3.5 mL/vial;_x000D_Streptavidin-HRP: Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;_x000D_Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;_x000D_Plate Seals (3): Adhesive strips

Description:

Product Overview

_x000D_

The Multiplex Rat Cytokine – Inflammation (9-Plex) is a fully quantitative ELISA-based Chemiluminescent assay allowing the concurrent measurement of nine biomarkers or analytes. Using just 25 µl of sample per well, up to 80 samples can be assayed for all nine markers in the panel within 3 hours.

The Multiplex Rat Cytokine – Inflammation (9-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing nine distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

_x000D_

Assay Ranges

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Analytes	Calibrator Range	Units	LLD
IFNγ	6000 – 8.2	pg/mL	5.3
IL-1α	5400 – 7.4	pg/mL	4.8
IL-1β	3200 – 4.4	pg/mL	2.9
IL-2	9800 – 13.4	pg/mL	8.7
IL-4	4000 – 5.5	pg/mL	3.6
IL-6	83000 – 113.9	pg/mL	74
IL-10	14000 – 19.2	pg/mL	12.5
IL-12p70	92000 – 126.2	pg/mL	82.0
TNFα	37000 – 50.8	pg/mL	33.0

_x000D_

Kit Components

_x000D_

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.

_x000D_

Reagents Include:

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Calibrator: Lyophilized, recombinant antigens in a buffered protein base;Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives ;Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide ;Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer ;Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives ;Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;Plate Seals (3): Adhesive strips

Description:

Product Overview

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The Multiplex Human Cytokine – Stripwells (16-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.25 hours.

The Multiplex Human Cytokine HS Screen (15-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

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Assay Ranges

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Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
IFNγ	Sandwich	8000 -10.97 (pg/mL)	5600 (pg/mL)	14.3 (pg/mL)	4.95 (pg/mL)
IL-1α	Sandwich	4000 -5.49 (pg/mL)	2800 (pg/mL)	7.1 (pg/mL)	5.43 (pg/mL)
IL-1β	Sandwich	12226 -16.77 (pg/mL)	8558 (pg/mL)	21.8 (pg/mL)	12.40 (pg/mL)
IL-2	Sandwich	5213 – 7.15 (pg/mL)	3649 (pg/mL)	9.3 (pg/mL)	4.12 (pg/mL)
IL-4	Sandwich	1676 – 2.30 (pg/mL)	1173 (pg/mL)	3.0 (pg/mL)	1.79 (pg/mL)
IL-5	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	2.90 (pg/mL)
IL-6	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	3.47 (pg/mL)
IL-8	Sandwich	2000 – 2.74 (pg/mL)	1400 (pg/mL)	3.6 (pg/mL)	1.37 (pg/mL)
IL-10	Sandwich	8039 – 11.03 (pg/mL)	5627 (pg/mL)	14.3 (pg/mL)	10.99 (pg/mL)
IL-12p70	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	3.20 (pg/mL)
IL-13	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	4.12 (pg/mL)
IL-15	Sandwich	10733 – 14.72 (pg/mL)	7513 (pg/mL)	19.1 (pg/mL)	6.87 (pg/mL)
IL-17A	Sandwich	16772 – 23.01 (pg/mL)	11740 (pg/mL)	29.9 (pg/mL)	22.57 (pg/mL)
IL-23	Sandwich	41700 – 57.20 (pg/mL)	21000 (pg/mL)	120 (pg/mL)	48.65 (pg/mL)
TNFα	Sandwich	6500 – 8.92 (pg/mL)	4522 (pg/mL)	11.5 (pg/mL)	7.74 (pg/mL)
TNFβ	Sandwich	4000 – 5.49 (pg/mL)	2800 (pg/mL)	7.1 (pg/mL)	5.29 (pg/mL)

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Kit Components

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Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.

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Reagents Include:

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Calibrator: Lyophilized, recombinant antigens in a buffered protein base;Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives;Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide;Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer;Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives ;Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;Plate Seals (3): Adhesive strips

Description:

Product Overview

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The Multiplex Mouse Cytokine Stripwells (15-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 15 biomarkers or analytes. Using just 25 µl of sample per well, up to 80 samples can be assayed for all 14 markers in the panel within 5.5 hours.

The Multiplex Mouse Cytokine Stripwells (15-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 15 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

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Assay Ranges

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Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
GMCSF	Sandwich	560 - 0.77 (pg/mL)	392 (pg/mL)	1 (pg/mL)	0.38 (pg/mL)
IFNγ	Sandwich	516 - 0.71 (pg/mL)	361 (pg/mL)	0.94 (pg/mL)	0.35 (pg/mL)
IL-1α	Sandwich	610 - 0.84 (pg/mL)	427 (pg/mL)	1.1 (pg/mL)	0.42 (pg/mL)
IL-1β	Sandwich	2500 - 3.43 (pg/mL)	1750 (pg/mL)	4.5 (pg/mL)	1.71 (pg/mL)
IL-2	Sandwich	360 - 0.49 (pg/mL)	252 (pg/mL)	0.6 (pg/mL)	0.25 (pg/mL)
IL-3	Sandwich	60 - 0.08 (pg/mL)	42 (pg/mL)	0.1 (pg/mL)	0.04 (pg/mL)
IL-4	Sandwich	400 - 0.55 (pg/mL)	280 (pg/mL)	0.7 (pg/mL)	0.27 (pg/mL)
IL-5	Sandwich	546 - 0.75 (pg/mL)	382 (pg/mL)	0.96 (pg/mL)	0.37 (pg/mL)
IL-6	Sandwich	504 - 0.69 (pg/mL)	353 (pg/mL)	0.91 (pg/mL)	0.35 (pg/mL)
IL-10	Sandwich	667 - 0.91 (pg/mL)	467 (pg/mL)	1.33 (pg/mL)	0.46 (pg/mL)
IL-12p70	Sandwich	572 - 0.78 (pg/mL)	400 (pg/mL)	1.01 (pg/mL)	0.39 (pg/mL)
IL-17A	Sandwich	760 - 1.04 (pg/mL)	532 (pg/mL)	1.4 (pg/mL)	0.52 (pg/mL)
MIP-1α	Sandwich	1000 - 1.37 (pg/mL)	700 (pg/mL)	1.8 (pg/mL)	0.69 (pg/mL)
RANTES	Sandwich	1653 - 2.27 (pg/mL)	1157 (pg/mL)	2.92 (pg/mL)	1.13 (pg/mL)
TNFα	Sandwich	429 - 0.59 (pg/mL)	300 (pg/mL)	0.69 (pg/mL)	0.29 (pg/mL)

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Kit Components

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Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.

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Reagents Include:

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Calibrator: Lyophilized, recombinant antigens in a buffered protein base;Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives ;Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide ;Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer ;Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives ;Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;Plate Seals (3): Adhesive strips