The melanocortin-5 receptor (mc5r) performs an essential position in exocrine operate, lipid metabolism, weight problems, and stress response within the vertebrate. Nonetheless, the capabilities of the mc5r in mollusks have been hardly ever investigated. We cloned the total size of Ruditapes philippinarum mc5r like gene (mc5rl) and the sequence construction and phylogenetic relationship of mc5rl have been analyzed. In addition to, we detected the tissue distribution and the expression sample of R. philippinarum mc5r like (mc5rl) genes after aerial publicity and low-temperature stress.
The complete-length cDNA of the mc5rl-1 was 2143 bp, consisting of a 1224 bp open studying body encoding (ORF) 408 amino acids. Sequence and phylogenetic analyses revealed that the nucleotide and amino acid sequences of Manila clam mc5rl have been extremely homologous with mc5r of Crassostrea virginica, Crassostrea gigas, Mizuhopecten yessoensis, and Pecten maximus (32%-36%) and low homologous with vertebrates. The outcomes of the distribution of mc5rl genes confirmed that mc5rl genes have been dominant within the mantle, gonad, and hepatopancreas in R. philippinarum. The expression of mc5rl genes was considerably elevated after aerial publicity and low-temperature stress in R. philippinarum in hepatopancreas. Aerial publicity and low-temperature stress might induce mc5rl expressed. Mc5rl may function a sensor and promote stress response in R. philippinarum.
The cloning and expression traits of mc5rl will facilitate the investigation of its operate in stress response and different physiological processes in R. philippinarum. Right here, the offered outcomes display that whereas superoxide dismutase genes remained reasonably fixed throughout long-term evolution, the whole rely of heme catalase genes was decreased in thermophilic fungi compared with their mesophilic counterparts. We display right here, for the newly found ascomycetous genes coding for thermophilic superoxide dismutases and catalases (originating from our sequencing challenge), the expression patterns of corresponding mRNA transcripts and additional analyze translated protein sequences. Our outcomes present essential implications for the physiology of reactive oxygen species metabolism in eukaryotic cells at elevated temperatures.
Genetic–molecular characterization within the prognosis of main immunodeficiencies
Targets: To rescue medical genetics ideas which can be crucial to grasp the advances within the genetic-molecular characterization of main immunodeficiencies, to assist in the understanding and sufficient interpretation of their outcomes.
Supply of knowledge: Non-systematic literature evaluation, looking for articles since 2000 on PubMed utilizing the phrases “genetic analysis” OR “complete exome sequence” or “complete genome sequence” OR “subsequent era sequence” AND “immunologic deficiency syndromes” OR “Immune deficiency illness” OR “immune deficiency” NOT HIV.
Abstract of the information: Data of medical genetics is crucial for the understanding of the ideas of heredity and illness inheritance patterns, varieties of genetic variants, varieties of genetic sequencing and interpretation of their outcomes. The medical and immunophenotypic analysis of every affected person is crucial for the correlation with the genetic variants noticed within the genetic research of sufferers with main immunodeficiencies. The dialogue of the advantages and limitations of genetic assessments ought to at all times information the efficiency of genetic assessments.
Conclusions: There are numerous evident advantages of genetic evaluation, such because the definitive prognosis of the illness, household genetic counseling, and the opportunity of a extra sufficient and correct administration. Value, entry and interpretation of genetic take a look at outcomes are limitations that want steady enchancment. The understanding of the advantages and limits of the a number of genetic evaluation methodologies associated to main immunodeficiencies is crucial to acquire more practical outcomes from the sequencing.
Molecular cloning and expression analysis of mc5r like genes (mc5rl) in Ruditapes philippinarum (Manila clam) after aerial exposure and low-temperature stress
Genetic variety research based mostly on morpho-pathological and molecular variability of the Sclerotinia sclerotiorum inhabitants infecting potato (Solanum tuberosum L.)
White mould or stem rot, brought on by Sclerotinia sclerotiorum (Lib.) de Bary, is a devastating fungal illness present in main potato cultivation areas worldwide. The intention of this research was to characterize genetic variety within the S. sclerotiorum inhabitants from the principle potato producing areas of India via morphological (mycelial development, colony color, quantity and distribution sample of sclerotia) and molecular traits, in addition to to guage the virulence of S. sclerotiorum isolates in potato for the primary time. Among the many S. sclerotiorum inhabitants analyzed, excessive phenotypic and genotypic variety have been noticed.
 Phenol Tris Equilibrated for molecular biology w/ Stabilizer |
|
12692 |
Sisco Laboratories |
60 ml |
EUR 10.13 |
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Description: Part A |
 Phenol Tris Equilibrated for molecular biology w/o Stabilizer |
|
78348 |
Sisco Laboratories |
100 ml |
EUR 13.75 |
|
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|
Description: Part A |
, 90%) Phenol Saturated w/ 10% water for molecular biology (Phenol Liquid w/ 10% water), 90% |
|
83275 |
Sisco Laboratories |
100 ml |
EUR 6.23 |
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Description: Part A |
 Phenol, crystalline |
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PB4112 |
Bio Basic |
500g |
EUR 210.34 |
|
|
 Acrylamide 3x cryst. for molecular biology, 99.9% |
|
61346 |
Sisco Laboratories |
100 Gms |
EUR 5.47 |
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|
Description: Part A |
 Phenol Equilibrated with 0.1M Citrate Buffer pH 4.5 for molecular biology w/ Stabilizer |
|
47484 |
Sisco Laboratories |
100 ml |
EUR 7.39 |
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Description: Part A |
 pH 4.5 for molecular biology) Phenol:Chloroform:Isoamyl Alcohol (125:24:1) pH 4.5 for molecular biology |
|
17080 |
Sisco Laboratories |
100 ml |
EUR 19.29 |
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Description: Part A |
 pH 8.0 for molecular biology) Phenol:Chloroform:Isoamyl Alcohol (25:24:1) pH 8.0 for molecular biology |
|
69031 |
Sisco Laboratories |
100 ml |
EUR 10.4 |
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Description: Part A |
 pH 6.7 for molecular biology) Phenol:Chloroform:Isoamyl Alcohol (49.5:49.5:1) pH 6.7 for molecular biology |
|
79030 |
Sisco Laboratories |
100 ml |
EUR 20.18 |
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Description: Part A |
 Phenol Crystalline pure, 99% |
|
28104 |
Sisco Laboratories |
500 Gms |
EUR 3.97 |
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Description: Part A |
 pH 8.0 for molecular biology) Phenol:Chloroform: Isoamyl Alcohol (49.5:49.5:1) pH 8.0 for molecular biology |
|
36976 |
Sisco Laboratories |
100 ml |
EUR 21.07 |
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Description: Part A |
 Phenol Crystalline extrapure AR, 99.5% |
|
14892 |
Sisco Laboratories |
500 Gms |
EUR 4.45 |
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Description: Part A |
 ex. Egg white (Muramidase) for molecular biology, 15000U/mg) Lysozyme (3x cryst) ex. Egg white (Muramidase) for molecular biology, 15000U/mg |
|
45822 |
Sisco Laboratories |
1 Gms |
EUR 6.3 |
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Description: Part B |
 Casein, for Molecular Biology |
|
MB279-100G |
EWC Diagnostics |
1 unit |
EUR 110.77 |
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Description: Casein, for Molecular Biology |
Casein, for Molecular Biology |
|
MB279-500G |
EWC Diagnostics |
1 unit |
EUR 387.65 |
|
Description: Casein, for Molecular Biology |
 N,N-Methylene Bisacrylamide 3x cryst. for molecular biology, 99.5% |
|
67320 |
Sisco Laboratories |
25 Gms |
EUR 3.97 |
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Description: Part A |
 Sucrose for molecular biology |
|
27580 |
Sisco Laboratories |
500 Gms |
EUR 4.11 |
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Description: Part A |
 Urea for molecular biology, 99.5% |
|
21113 |
Sisco Laboratories |
500 Gms |
EUR 6.5 |
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Description: Part A |
 Xylene for molecular biology, 99.5% |
|
45122 |
Sisco Laboratories |
250 ml |
EUR 2.81 |
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Description: Part A |
 Glycine for molecular biology, 99.5% |
|
64072 |
Sisco Laboratories |
100 Gms |
EUR 1.64 |
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Description: Part A |
 Acetone for molecular biology, 99.8% |
|
27498 |
Sisco Laboratories |
500 ml |
EUR 3.76 |
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Description: Part A |
 Methanol for molecular biology, 99.5% |
|
96446 |
Sisco Laboratories |
500 ml |
EUR 6.23 |
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Description: Part A |
 Carbinol for molecular biology, 99.5% |
|
34883 |
Sisco Laboratories |
500 ml |
EUR 6.23 |
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Description: Part A |
 Formamide for molecular biology, 99.5% |
|
30349 |
Sisco Laboratories |
500 ml |
EUR 5.82 |
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Description: Part A |
 Imidazole for molecular biology, 99.5% |
|
61510 |
Sisco Laboratories |
25 Gms |
EUR 5.47 |
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Description: Part A |
 Chloroform for molecular biology, 99.8% |
|
96764 |
Sisco Laboratories |
100 ml |
EUR 3.08 |
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Description: Part A |
 Triethylamine for molecular biology, 99.5% |
|
55221 |
Sisco Laboratories |
500 ml |
EUR 4.11 |
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Description: Part A |
 Ficoll 400® for molecular biology |
|
45460 |
Sisco Laboratories |
5 Gms |
EUR 15.6 |
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Description: Part B |
 Agarose UltraPhor for molecular biology |
|
27813 |
Sisco Laboratories |
10 Gms |
EUR 46.5 |
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Description: Part B |
 Boric Acid for molecular biology, 99.5% |
|
22311 |
Sisco Laboratories |
250 Gms |
EUR 4.04 |
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Description: Part A |
 2-Mercaptoethanol ?For Molecular Biology |
|
MB041-100ML |
EWC Diagnostics |
1 unit |
EUR 9.02 |
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Description: 2-Mercaptoethanol ?For Molecular Biology |
2-Mercaptoethanol ?For Molecular Biology |
|
MB041-500ML |
EWC Diagnostics |
1 unit |
EUR 26.47 |
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Description: 2-Mercaptoethanol ?For Molecular Biology |
 Triton X-100 for molecular biology |
|
64518 |
Sisco Laboratories |
100 ml |
EUR 6.02 |
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Description: Part A |
 MOPS Buffer for molecular biology, 99% |
|
66043 |
Sisco Laboratories |
25 Gms |
EUR 10.28 |
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Description: Part B |
 ACES Buffer for molecular biology, 99.5% |
|
27907 |
Sisco Laboratories |
5 Gms |
EUR 5.4 |
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Description: Part B |
 Vitamin B12 for molecular biology, 97% |
|
77472 |
Sisco Laboratories |
250 Mg |
EUR 5.78 |
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Description: Part B |
 Agarose Low EEO for molecular biology |
|
36601 |
Sisco Laboratories |
10 Gms |
EUR 5.1 |
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Description: Part B |
 10X MOPS Buffer for molecular biology |
|
68078 |
Sisco Laboratories |
100 ml |
EUR 4.5 |
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Description: Part B |
 CHAPS Buffer for molecular biology, 99.5% |
|
21420 |
Sisco Laboratories |
1 Gms |
EUR 10.73 |
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Description: Part B |
 HEPES Buffer for molecular biology, 99.5% |
|
16826 |
Sisco Laboratories |
25 Gms |
EUR 6.3 |
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Description: Part B |
 Sodium Azide for molecular biology, 99% |
|
17782 |
Sisco Laboratories |
100 Gms |
EUR 5.47 |
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Description: Part A |
 PIPES Buffer for molecular biology, 99% |
|
49159 |
Sisco Laboratories |
100 Gms |
EUR 26.03 |
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Description: Part B |
 for molecular biology, 99%) Phenylmethane Sulphonyl Fluoride (PMSF) for molecular biology, 99% |
|
84375 |
Sisco Laboratories |
1 Gms |
EUR 7.13 |
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Description: Part B |
 Agarose High EEO for molecular biology |
|
60645 |
Sisco Laboratories |
25 Gms |
EUR 13.8 |
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Description: Part B |
 Jasmonic Acid for molecular biology, 95% |
|
79238 |
Sisco Laboratories |
25 Mg |
EUR 120.9 |
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Description: Part B |
 Protamine Sulfate for molecular biology, 90-110% |
|
78349 |
Sisco Laboratories |
1 Gms |
EUR 22.5 |
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Description: Part B |
 Oil Red O for molecular biology, 75% |
|
23576 |
Sisco Laboratories |
25 Gms |
EUR 5.33 |
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Description: Part B |
 Sodium Chloride for molecular biology, 99.9% |
|
33205 |
Sisco Laboratories |
500 Gms |
EUR 4.52 |
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Description: Part A |
 Agarose Medium EEO for molecular biology |
|
10423 |
Sisco Laboratories |
10 Gms |
EUR 6.75 |
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Description: Part B |
 for molecular biology, 99.8%) Isopropanol (IPA) for molecular biology, 99.8% |
|
38445 |
Sisco Laboratories |
100 ml |
EUR 1.71 |
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Description: Part A |
 for molecular biology, 99.9%) Acetonitrile (ACN) for molecular biology, 99.9% |
|
62006 |
Sisco Laboratories |
250 ml |
EUR 4.93 |
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Description: Part A |
 Ethidium Bromide for molecular biology, 95% |
|
93079 |
Sisco Laboratories |
1 Gms |
EUR 4.58 |
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Description: Part A |
 Ammonium Acetate for molecular biology, 98% |
|
37829 |
Sisco Laboratories |
100 Gms |
EUR 2.12 |
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Description: Part A |
 Agarose Low Melting for molecular biology |
|
32417 |
Sisco Laboratories |
5 Gms |
EUR 22.5 |
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Description: Part B |
 Potassium Acetate for molecular biology, 99.5% |
|
96248 |
Sisco Laboratories |
100 Gms |
EUR 2.74 |
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Description: Part A |
 Ammonium Chloride for molecular biology, 99.5% |
|
16992 |
Sisco Laboratories |
500 Gms |
EUR 4.52 |
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Description: Part A |
 Ammonium Sulphate for molecular biology, 99.5% |
|
82126 |
Sisco Laboratories |
250 Gms |
EUR 4.93 |
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Description: Part A |
 Potassium Chloride for molecular biology, 99.5% |
|
84984 |
Sisco Laboratories |
500 Gms |
EUR 6.16 |
|
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Description: Part A |
 Sodium Bicarbonate for molecular biology, 99.7% |
|
36328 |
Sisco Laboratories |
500 Gms |
EUR 5.47 |
|
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Description: Part A |
 for molecular biology, 99.5%) Glycerol (Glycerine) for molecular biology, 99.5% |
|
62417 |
Sisco Laboratories |
100 ml |
EUR 2.05 |
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Description: Part A |
 2-Mercaptoethanol for molecular biology, 99% |
|
83759 |
Sisco Laboratories |
100 ml |
EUR 5.27 |
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Description: Part A |
 Acriflavine Neutral for molecular biology, 98% |
|
42428 |
Sisco Laboratories |
5 Gms |
EUR 3.15 |
|
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Description: Part B |
 for molecular biology, 99.9%) Dichloromethane (DCM) for molecular biology, 99.9% |
|
41512 |
Sisco Laboratories |
250 ml |
EUR 3.22 |
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Description: Part A |
 for molecular biology, 99.8%) Tetrahydrofuran (THF) for molecular biology, 99.8% |
|
92562 |
Sisco Laboratories |
250 ml |
EUR 3.28 |
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Description: Part A |
 for molecular biology, 99%) Dithioerythritol (DTE) for molecular biology, 99% |
|
53384 |
Sisco Laboratories |
1 Gms |
EUR 9.53 |
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Description: Part B |
 for molecular biology, 98%) meta-Topolin (mT) for molecular biology, 98% |
|
68913 |
Sisco Laboratories |
25 Mg |
EUR 17.48 |
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Description: Part B |
 L-Ascorbic Acid for molecular biology, 99.7% |
|
14116 |
Sisco Laboratories |
25 Gms |
EUR 1.64 |
|
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Description: Part A |
 HEPES Sodium Salt for molecular biology, 99% |
|
32225 |
Sisco Laboratories |
25 Gms |
EUR 11.85 |
|
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Description: Part B |
 Glycogen ex. Oyster for molecular biology, 85% |
|
49740 |
Sisco Laboratories |
1 Gms |
EUR 7.5 |
|
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Description: Part B |
 D-Sorbitol Powder for molecular biology, 98% |
|
99103 |
Sisco Laboratories |
500 Gms |
EUR 10.33 |
|
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Description: Part A |
 Acetic Acid Glacial for molecular biology, 99.9% |
|
59788 |
Sisco Laboratories |
100 ml |
EUR 3.56 |
|
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Description: Part A |
 Brilliant Blue G-250 for molecular biology |
|
64222 |
Sisco Laboratories |
5 Gms |
EUR 4.65 |
|
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Description: Part B |
 Brilliant Blue R-250 for molecular biology |
|
93473 |
Sisco Laboratories |
5 Gms |
EUR 4.35 |
|
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Description: Part B |
 for molecular biology, 95%) Propidium Iodide (PI) for molecular biology, 95% |
|
11195 |
Sisco Laboratories |
10 Mg |
EUR 17.63 |
|
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Description: Part B |
 Acriflavine Hydrochloride for molecular biology, 98.5%-105% |
|
35188 |
Sisco Laboratories |
5 Gms |
EUR 4.05 |
|
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Description: Part B |
 for molecular biology) Polysorbate 20 (Tween 20) for molecular biology |
|
65296 |
Sisco Laboratories |
100 Gms |
EUR 10.81 |
|
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Description: Part A |
 Tris Buffer Superior for molecular biology, 99.9% |
|
37969 |
Sisco Laboratories |
100 Gms |
EUR 7.59 |
|
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Description: Part A |
 Molecular Biology Grade Water for RT-PCR |
|
ML065-1.5ML |
EWC Diagnostics |
1 unit |
EUR 7.82 |
|
Description: Molecular Biology Grade Water for RT-PCR |
 10X TE Buffer pH-8.0 for molecular biology |
|
51782 |
Sisco Laboratories |
100 ml |
EUR 9.45 |
|
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Description: Part B |
 10X TBE Buffer pH-8.3 for molecular biology |
|
83170 |
Sisco Laboratories |
200 ml |
EUR 4.95 |
|
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Description: Part B |
 Tris Buffer AR, ACS for molecular biology, 99.9% |
|
71033 |
Sisco Laboratories |
100 Gms |
EUR 6.02 |
|
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Description: Part A |
 10X TAE Buffer pH-8.3 for molecular biology |
|
71989 |
Sisco Laboratories |
100 ml |
EUR 4.43 |
|
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Description: Part B |
 Acrylamide 40% aq. solution for molecular biology |
|
29787 |
Sisco Laboratories |
250 ml |
EUR 20.53 |
|
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Description: Part A |
 MES Monohydrate Buffer for molecular biology, 99% |
|
26854 |
Sisco Laboratories |
25 Gms |
EUR 11.03 |
|
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Description: Part B |
 for molecular biology, 99.5%) Glutathione Reduced (GSH) for molecular biology, 99.5% |
|
11514 |
Sisco Laboratories |
1 Gms |
EUR 5.55 |
|
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Description: Part B |
 for molecular biology, 98%) DL-Dithiothreitol (DTT) for molecular biology, 98% |
|
17315 |
Sisco Laboratories |
1 Gms |
EUR 5.18 |
|
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Description: Part B |
 for molecular biology, 99.9%) Trifluoroacetic Acid (TFA) for molecular biology, 99.9% |
|
40801 |
Sisco Laboratories |
100 ml |
EUR 22.92 |
|
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Description: Part A |
 for molecular biology, 99%) Ammonium Persulphate (APS) for molecular biology, 99% |
|
65553 |
Sisco Laboratories |
25 Gms |
EUR 1.64 |
|
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Description: Part A |
 Sodium Acetate Anhydrous for molecular biology, 99% |
|
22342 |
Sisco Laboratories |
250 Gms |
EUR 3.56 |
|
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Description: Part A |
 for molecular biology, 99.8%) Dimethyl Sulphoxide (DMSO) for molecular biology, 99.8% |
|
24075 |
Sisco Laboratories |
100 ml |
EUR 6.02 |
|
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Description: Part A |
 Sodium Acetate Trihydrate for molecular biology, 99.5% |
|
88035 |
Sisco Laboratories |
250 Gms |
EUR 2.33 |
|
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Description: Part A |
 Cesium Sulphate extrapure for molecular biology, 99.9% |
|
57455 |
Sisco Laboratories |
10 Gms |
EUR 7.5 |
|
|
|
Description: Part B |
 for molecular biology, 99.5%) Glutathione Oxidized (GSSG) for molecular biology, 99.5% |
|
22151 |
Sisco Laboratories |
250 Mg |
EUR 22.5 |
|
|
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Description: Part B |
 for molecular biology, 99%) Guanidine Thiocyanate (GTC) for molecular biology, 99% |
|
80272 |
Sisco Laboratories |
100 Gms |
EUR 5.47 |
|
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Description: Part A |
 Cesium Chloride ultrapure for molecular biology, 99.9% |
|
32355 |
Sisco Laboratories |
25 Gms |
EUR 18 |
|
|
|
Description: Part B |
 Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve |
|
GS8780 |
Glentham Life Sciences |
2500ml |
EUR 239.63 |
Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve |
|
GS8780-2500 |
Glentham Life Sciences |
2500 |
EUR 249.8 |
|
|
 Calcium Chloride Dihydrate for molecular biology, 99.5% |
|
97080 |
Sisco Laboratories |
100 Gms |
EUR 2.12 |
|
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|
Description: Part A |
 for molecular biology, 99%) Diethyl Pyrocarbonate (DEPC) for molecular biology, 99% |
|
46791 |
Sisco Laboratories |
5 ml |
EUR 9 |
|
|
|
Description: Part B |
 Phenol Crystalline extrapure AR, ACS, ExiPlus, Multi-Compendial, 99.5% |
|
97745 |
Sisco Laboratories |
500 Gms |
EUR 4.93 |
|
|
|
Description: Part A |
 20X SSC Buffer pH 6.9-7.1 for molecular biology |
|
12590 |
Sisco Laboratories |
200 ml |
EUR 2.48 |
|
|
|
Description: Part B |
 Bromocresol Purple Sodium Salt for molecular biology |
|
24836 |
Sisco Laboratories |
5 Gms |
EUR 2.05 |
|
|
|
Description: Part A |
 for molecular biology, 99.5%) Guanidine Hydrochloride (GHC) for molecular biology, 99.5% |
|
45539 |
Sisco Laboratories |
100 Gms |
EUR 4.79 |
|
|
|
Description: Part A |
 for molecular biology) Polyethylene Glycol 8000 (PEG 8000) for molecular biology |
|
54866 |
Sisco Laboratories |
500 Gms |
EUR 11.97 |
|
|
|
Description: Part A |
 for molecular biology) 10X Tris Buffered Saline (TBS) for molecular biology |
|
83471 |
Sisco Laboratories |
500 ml |
EUR 14.7 |
|
|
|
Description: Part B |
 Magnesium Acetate Tetrahydrate for molecular biology, 99% |
|
50488 |
Sisco Laboratories |
100 Gms |
EUR 2.74 |
|
|
|
Description: Part A |
 Magnesium Chloride Hexahydrate for molecular biology, 99.5% |
|
91417 |
Sisco Laboratories |
250 Gms |
EUR 4.45 |
|
|
|
Description: Part A |
 Magnesium Sulphate Heptahydrate for molecular biology, 99.5% |
|
74440 |
Sisco Laboratories |
500 Gms |
EUR 6.16 |
|
|
|
Description: Part A |
 for molecular biology, 99%) Tris Hydrochloride (Tris HCl) for molecular biology, 99% |
|
89781 |
Sisco Laboratories |
100 Gms |
EUR 9.37 |
|
|
|
Description: Part A |
 for molecular biology, 99%) L-Lysine Monohydrate (base) for molecular biology, 99% |
|
45976 |
Sisco Laboratories |
25 Gms |
EUR 37.63 |
|
|
|
Description: Part A |
 Agarose Low EEO Superior Grade for molecular biology |
|
23287 |
Sisco Laboratories |
10 Gms |
EUR 6 |
|
|
|
Description: Part B |
) DTT (Molecular Biology Grade) |
|
CE131 |
GeneOn |
5 g |
EUR 44 |
DTT (Molecular Biology Grade) |
|
CE132 |
GeneOn |
10 g |
EUR 88 |
DTT (Molecular Biology Grade) |
|
CE133 |
GeneOn |
25 g |
EUR 170 |
) NAD (Molecular Biology Grade) |
|
CE196 |
GeneOn |
1 g |
EUR 34 |
NAD (Molecular Biology Grade) |
|
CE197 |
GeneOn |
5 g |
EUR 137 |
Utilizing all of the morphological traits, a dendrogram was constructed based mostly on Gower’s similarity coefficient that distributed all of the isolates into three clusters on the 0.62 similarity coefficient. Carpogenic germination of apothecia revealed that bigger sclerotia produced a better variety of apothecia whereas smaller sclerotia produced fewer apothecia. Pathogenicity take a look at outcomes revealed that out of 25 isolates, seven have been extremely aggressive, 14 have been reasonable and 4 had low aggressiveness, while isolates from Punjab have been extra pathogenic than these of Uttar Pradesh. Phylogenetic evaluation of common rice primer polymorphism confirmed excessive genetic variability throughout the isolates that grouped all of the isolates in three evolutionary lineages within the ensuing dendrogram and confirmed partial relationship with geographical areas of the isolates. Additional, the findings counsel the prevalence of upper heterogeneity and genetic variety among the many S. sclerotiorum isolates that signifies the existence of each clonal and sexual copy within the pathogen inhabitants of potato producing areas in India.
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