The melanocortin-5 receptor (mc5r) performs an essential position in exocrine operate, lipid metabolism, weight problems, and stress response within the vertebrate. Nonetheless, the capabilities of the mc5r in mollusks have been hardly ever investigated. We cloned the total size of Ruditapes philippinarum mc5r like gene (mc5rl) and the sequence construction and phylogenetic relationship of mc5rl have been analyzed. In addition to, we detected the tissue distribution and the expression sample of R. philippinarum mc5r like (mc5rl) genes after aerial publicity and low-temperature stress.
The complete-length cDNA of the mc5rl-1 was 2143 bp, consisting of a 1224 bp open studying body encoding (ORF) 408 amino acids. Sequence and phylogenetic analyses revealed that the nucleotide and amino acid sequences of Manila clam mc5rl have been extremely homologous with mc5r of Crassostrea virginica, Crassostrea gigas, Mizuhopecten yessoensis, and Pecten maximus (32%-36%) and low homologous with vertebrates. The outcomes of the distribution of mc5rl genes confirmed that mc5rl genes have been dominant within the mantle, gonad, and hepatopancreas in R. philippinarum. The expression of mc5rl genes was considerably elevated after aerial publicity and low-temperature stress in R. philippinarum in hepatopancreas. Aerial publicity and low-temperature stress might induce mc5rl expressed. Mc5rl may function a sensor and promote stress response in R. philippinarum.
The cloning and expression traits of mc5rl will facilitate the investigation of its operate in stress response and different physiological processes in R. philippinarum. Right here, the offered outcomes display that whereas superoxide dismutase genes remained reasonably fixed throughout long-term evolution, the whole rely of heme catalase genes was decreased in thermophilic fungi compared with their mesophilic counterparts. We display right here, for the newly found ascomycetous genes coding for thermophilic superoxide dismutases and catalases (originating from our sequencing challenge), the expression patterns of corresponding mRNA transcripts and additional analyze translated protein sequences. Our outcomes present essential implications for the physiology of reactive oxygen species metabolism in eukaryotic cells at elevated temperatures.
Genetic–molecular characterization within the prognosis of main immunodeficiencies
Targets: To rescue medical genetics ideas which can be crucial to grasp the advances within the genetic-molecular characterization of main immunodeficiencies, to assist in the understanding and sufficient interpretation of their outcomes.
Supply of knowledge: Non-systematic literature evaluation, looking for articles since 2000 on PubMed utilizing the phrases “genetic analysis” OR “complete exome sequence” or “complete genome sequence” OR “subsequent era sequence” AND “immunologic deficiency syndromes” OR “Immune deficiency illness” OR “immune deficiency” NOT HIV.
Abstract of the information: Data of medical genetics is crucial for the understanding of the ideas of heredity and illness inheritance patterns, varieties of genetic variants, varieties of genetic sequencing and interpretation of their outcomes. The medical and immunophenotypic analysis of every affected person is crucial for the correlation with the genetic variants noticed within the genetic research of sufferers with main immunodeficiencies. The dialogue of the advantages and limitations of genetic assessments ought to at all times information the efficiency of genetic assessments.
Conclusions: There are numerous evident advantages of genetic evaluation, such because the definitive prognosis of the illness, household genetic counseling, and the opportunity of a extra sufficient and correct administration. Value, entry and interpretation of genetic take a look at outcomes are limitations that want steady enchancment. The understanding of the advantages and limits of the a number of genetic evaluation methodologies associated to main immunodeficiencies is crucial to acquire more practical outcomes from the sequencing.
Molecular cloning and expression analysis of mc5r like genes (mc5rl) in Ruditapes philippinarum (Manila clam) after aerial exposure and low-temperature stress
Genetic variety research based mostly on morpho-pathological and molecular variability of the Sclerotinia sclerotiorum inhabitants infecting potato (Solanum tuberosum L.)
White mould or stem rot, brought on by Sclerotinia sclerotiorum (Lib.) de Bary, is a devastating fungal illness present in main potato cultivation areas worldwide. The intention of this research was to characterize genetic variety within the S. sclerotiorum inhabitants from the principle potato producing areas of India via morphological (mycelial development, colony color, quantity and distribution sample of sclerotia) and molecular traits, in addition to to guage the virulence of S. sclerotiorum isolates in potato for the primary time. Among the many S. sclerotiorum inhabitants analyzed, excessive phenotypic and genotypic variety have been noticed.
) NAD (Molecular Biology Grade) |
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CE196 |
GeneOn |
1 g |
EUR 72 |
NAD (Molecular Biology Grade) |
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CE197 |
GeneOn |
5 g |
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CE209 |
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1 g |
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NBT (Molecular Biology Grade) |
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CE210 |
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5 g |
EUR 360 |
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CE131 |
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5 g |
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DTT (Molecular Biology Grade) |
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CE132 |
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10 g |
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DTT (Molecular Biology Grade) |
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CE133 |
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25 g |
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CE237 |
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500 g |
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Tris (Molecular Biology Grade) |
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CE238 |
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1 kg |
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Tris (Molecular Biology Grade) |
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CE239 |
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5 kg |
EUR 535.2 |
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CE108 |
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250 mg |
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BCIP (Molecular Biology Grade) |
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CE109 |
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1 g |
EUR 108 |
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CE117 |
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DAPI (Molecular Biology Grade) |
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CE118 |
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25 mg |
EUR 159.6 |
DAPI (Molecular Biology Grade) |
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CE119 |
GeneOn |
100 mg |
EUR 382.8 |
 DMSO, Molecular Biology Grade |
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40470006-1 |
Bio-WORLD |
100 mL |
EUR 88.18 |
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DMSO, Molecular Biology Grade |
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40470006-2 |
Bio-WORLD |
250 mL |
EUR 150.19 |
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DMSO, Molecular Biology Grade |
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40470006-3 |
Bio-WORLD |
500 mL |
EUR 279.26 |
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 EGTA, Molecular Biology Grade |
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40500028-2 |
Bio-WORLD |
50 g |
EUR 106.43 |
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EGTA, Molecular Biology Grade |
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40500028-3 |
Bio-WORLD |
100 g |
EUR 177.58 |
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EGTA, Molecular Biology Grade |
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40500028-4 |
Bio-WORLD |
500 g |
EUR 603.19 |
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EGTA, Molecular Biology Grade |
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40500028-5 |
Bio-WORLD |
1 kg |
EUR 912.98 |
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EGTA, Molecular Biology Grade |
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40500028-6 |
Bio-WORLD |
2 kg |
EUR 1687.94 |
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) HEPES (Molecular Biology Grade) |
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CE171 |
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100 g |
EUR 98.4 |
HEPES (Molecular Biology Grade) |
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CE172 |
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500 g |
EUR 268.8 |
HEPES (Molecular Biology Grade) |
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CE173 |
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1 kg |
EUR 424.8 |
) Water (Molecular Biology Grade) |
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CE243 |
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500 ml |
EUR 62.4 |
Water (Molecular Biology Grade) |
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CE244 |
GeneOn |
1 l |
EUR 67.2 |
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CE114 |
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1 g |
EUR 66 |
CHAPS (Molecular Biology Grade) |
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CE115 |
GeneOn |
5 g |
EUR 157.2 |
CHAPS (Molecular Biology Grade) |
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CE116 |
GeneOn |
25 g |
EUR 492 |
 Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve |
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GS8780-2500 |
Glentham Life Sciences |
2500 |
EUR 249.8 |
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) Tween20 (Molecular Biology Grade) |
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CE242 |
GeneOn |
1 l |
EUR 106.8 |
) Glycine (Molecular Biology Grade) |
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CE158 |
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1 kg |
EUR 84 |
Glycine (Molecular Biology Grade) |
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CE159 |
GeneOn |
5 kg |
EUR 228 |
 Agarose, Molecular Biology Grade |
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40100164-1 |
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25 g |
Ask for price |
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Agarose, Molecular Biology Grade |
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40100164-2 |
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50 g |
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Agarose, Molecular Biology Grade |
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40100164-3 |
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100 g |
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Agarose, Molecular Biology Grade |
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40100164-4 |
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500 g |
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Agarose, Molecular Biology Grade |
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40100164-5 |
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1 kg |
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) Lysozyme (Molecular Biology Grade) |
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CE188 |
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1 g |
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Lysozyme (Molecular Biology Grade) |
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CE189 |
GeneOn |
10 g |
EUR 247.2 |
 Tween 20, Molecular Biology Grade |
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T9100-010 |
GenDepot |
100ml |
EUR 86.4 |
Tween 20, Molecular Biology Grade |
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T9100-050 |
GenDepot |
500ml |
EUR 133.2 |
Tween 20, Molecular Biology Grade |
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T9100-100 |
GenDepot |
1L |
EUR 160.8 |
 Dimethylformamide, GlenBiol™, suitable for molecular biology with molecular sieve |
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GS3406-2500 |
Glentham Life Sciences |
2500 |
EUR 116.2 |
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-Sucrose (Molecular Biology Grade)) D(+)-Sucrose (Molecular Biology Grade) |
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CE224 |
GeneOn |
500 g |
EUR 67.2 |
D(+)-Sucrose (Molecular Biology Grade) |
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CE225 |
GeneOn |
1 kg |
EUR 84 |
D(+)-Sucrose (Molecular Biology Grade) |
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CE226 |
GeneOn |
5 kg |
EUR 207.6 |
 Boric Acid, Molecular Biology Grade |
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40200060-1 |
Bio-WORLD |
500 g |
EUR 46.16 |
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Boric Acid, Molecular Biology Grade |
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40200060-2 |
Bio-WORLD |
1 kg |
EUR 76.66 |
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Boric Acid, Molecular Biology Grade |
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40200060-3 |
Bio-WORLD |
2.5 kg |
EUR 145.5 |
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) MOPS buffer (Molecular Biology Grade) |
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CE194 |
GeneOn |
100 g |
EUR 102 |
MOPS buffer (Molecular Biology Grade) |
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CE195 |
GeneOn |
250 g |
EUR 169.2 |
) Glycerol 87 % (Molecular Biology Grade) |
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CE154 |
GeneOn |
1 l |
EUR 93.6 |
) Formamide deionized (Molecular Biology Grade) |
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CE145 |
GeneOn |
500 ml |
EUR 87.6 |
Formamide deionized (Molecular Biology Grade) |
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CE146 |
GeneOn |
1 l |
EUR 120 |
 Dimethylformamide, GlenBiol™, suitable for molecular biology |
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GS6580-2500 |
Glentham Life Sciences |
2500 |
EUR 107.3 |
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) TritonX-100 (Molecular Biology Grade) |
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CE240 |
GeneOn |
500 ml |
EUR 67.2 |
TritonX-100 (Molecular Biology Grade) |
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CE241 |
GeneOn |
1 l |
EUR 79.2 |
) Dimethylsulfoxide (Molecular Biology Grade) |
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CE120 |
GeneOn |
100 ml |
EUR 66 |
Dimethylsulfoxide (Molecular Biology Grade) |
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CE121 |
GeneOn |
500 ml |
EUR 110.4 |
 Water, Ultrapure Molecular Biology Grade |
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41024-4L |
Biotium |
4L |
EUR 145.2 |
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Description: Minimum order quantity: 1 unit of 4L |
) Sodium chloride (Molecular Biology Grade) |
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CE205 |
GeneOn |
500 g |
EUR 62.4 |
Sodium chloride (Molecular Biology Grade) |
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CE206 |
GeneOn |
1 kg |
EUR 70.8 |
Sodium chloride (Molecular Biology Grade) |
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CE207 |
GeneOn |
5 kg |
EUR 123.6 |
) Bis-Acrylamid (Molecular Biology Grade) |
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CE110 |
GeneOn |
50 g |
EUR 94.8 |
Bis-Acrylamid (Molecular Biology Grade) |
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CE111 |
GeneOn |
250 g |
EUR 259.2 |
 Cesium Chloride, Molecular Biology Grade |
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40300060-1 |
Bio-WORLD |
50 g |
EUR 45.77 |
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) Ammonium sulfate (Molecular Biology Grade) |
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CE105 |
GeneOn |
250 g |
EUR 55.2 |
Ammonium sulfate (Molecular Biology Grade) |
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CE106 |
GeneOn |
1 kg |
EUR 72 |
Ammonium sulfate (Molecular Biology Grade) |
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CE107 |
GeneOn |
5 kg |
EUR 153.6 |
 Lithium Chloride, Molecular Biology Grade |
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41200036-1 |
Bio-WORLD |
100 g |
EUR 41.7 |
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Lithium Chloride, Molecular Biology Grade |
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41200036-2 |
Bio-WORLD |
500 g |
EUR 108.38 |
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 (Molecular Biology Grade)) SSC Buffer (20X) (Molecular Biology Grade) |
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CE229 |
GeneOn |
1 l |
EUR 86.4 |
) XTT sodium salt (Molecular Biology Grade) |
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CE250 |
GeneOn |
100 mg |
EUR 208.8 |
XTT sodium salt (Molecular Biology Grade) |
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CE251 |
GeneOn |
500 mg |
EUR 612 |
) Tris - Hydrochloride (Molecular Biology Grade) |
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CE234 |
GeneOn |
250 g |
EUR 99.6 |
Tris - Hydrochloride (Molecular Biology Grade) |
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CE235 |
GeneOn |
500 g |
EUR 144 |
Tris - Hydrochloride (Molecular Biology Grade) |
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CE236 |
GeneOn |
1 kg |
EUR 223.2 |
) Glycerol waterfree (Molecular Biology Grade) |
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CE155 |
GeneOn |
500 ml |
EUR 78 |
Glycerol waterfree (Molecular Biology Grade) |
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CE156 |
GeneOn |
1 l |
EUR 102 |
Glycerol waterfree (Molecular Biology Grade) |
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CE157 |
GeneOn |
2.5 l |
EUR 170.4 |
) NADP - sodium salt (Molecular Biology Grade) |
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CE200 |
GeneOn |
250 mg |
EUR 92.4 |
NADP - sodium salt (Molecular Biology Grade) |
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CE201 |
GeneOn |
1 g |
EUR 190.8 |
) NADH - Disodium salt (Molecular Biology Grade) |
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CE198 |
GeneOn |
1 g |
EUR 91.2 |
NADH - Disodium salt (Molecular Biology Grade) |
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CE199 |
GeneOn |
5 g |
EUR 244.8 |
) Guanidine Thiocyanate (Molecular Biology Grade) |
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CE164 |
GeneOn |
100 g |
EUR 86.4 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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CE165 |
GeneOn |
500 g |
EUR 192 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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CE166 |
GeneOn |
1 kg |
EUR 307.2 |
) Guanidine - Hydrochloride (Molecular Biology Grade) |
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CE163 |
GeneOn |
1 kg |
EUR 352.8 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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CE160 |
GeneOn |
100 g |
EUR 93.6 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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CE161 |
GeneOn |
250 g |
EUR 153.6 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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CE162 |
GeneOn |
500 g |
EUR 232.8 |
) Yeast extract powder (Molecular Biology Grade) |
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CE169 |
GeneOn |
500 g |
EUR 133.2 |
-Glucose waterfree (Molecular Biology Grade)) D(+)-Glucose waterfree (Molecular Biology Grade) |
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CE148 |
GeneOn |
500 g |
EUR 67.2 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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CE149 |
GeneOn |
1 kg |
EUR 75.6 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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CE150 |
GeneOn |
5 kg |
EUR 180 |
) Hyaluronidase Grade I (Molecular Biology Grade) |
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CE174 |
GeneOn |
1 g |
EUR 232.8 |
Hyaluronidase Grade I (Molecular Biology Grade) |
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CE175 |
GeneOn |
5 g |
EUR 920.4 |
) NADPH - Tetrasodium salt (Molecular Biology Grade) |
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CE202 |
GeneOn |
25 mg |
EUR 70.8 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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CE203 |
GeneOn |
100 mg |
EUR 126 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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CE204 |
GeneOn |
500 mg |
EUR 374.4 |
 TRIS-Glycine Buffer 10X, GlenBiol™, suitable for molecular biology |
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GE6710-250 |
Glentham Life Sciences |
250 |
EUR 22.7 |
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 Di-iso-propylethylamine, GlenBiol™, suitable for molecular biology |
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GS7181-100 |
Glentham Life Sciences |
100 |
EUR 143.3 |
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Utilizing all of the morphological traits, a dendrogram was constructed based mostly on Gower’s similarity coefficient that distributed all of the isolates into three clusters on the 0.62 similarity coefficient. Carpogenic germination of apothecia revealed that bigger sclerotia produced a better variety of apothecia whereas smaller sclerotia produced fewer apothecia. Pathogenicity take a look at outcomes revealed that out of 25 isolates, seven have been extremely aggressive, 14 have been reasonable and 4 had low aggressiveness, while isolates from Punjab have been extra pathogenic than these of Uttar Pradesh. Phylogenetic evaluation of common rice primer polymorphism confirmed excessive genetic variability throughout the isolates that grouped all of the isolates in three evolutionary lineages within the ensuing dendrogram and confirmed partial relationship with geographical areas of the isolates. Additional, the findings counsel the prevalence of upper heterogeneity and genetic variety among the many S. sclerotiorum isolates that signifies the existence of each clonal and sexual copy within the pathogen inhabitants of potato producing areas in India.
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