Nitric oxide (NO) is a second messenger that regulates various physiological processes in plants. NO-derived molecules called reactive nitrogen species (RNS) can react with unsaturated fatty acids produces fatty acids nitration (NO2-FA). NO2-FA work as a signaling molecule in mammals in which the production and the target has been described under different stress conditions. More recently, NO2-fatty acids were detected in plants, but their role (s) on plant physiological processes is still poorly known. Although in this work NO2-OA has not been detected in any tissue of Arabidopsis seedlings, here we show that the application of exogenous growth (NO2-OA) inhibits Arabidopsis primary root nitro-oleic acid;
This inhibition is not possible due to the production of oxide (NO) nitrate or disorder root auxin or cytokinin response. In-depth analysis shows that the roots were incubated with NO2-OA has a lower number of cells in the division. Although NO2-FA does not affect hormonal signaling mechanisms maintain the stem cell niche, the plants were incubated with NO2-OA showed a decrease in cell division in the meristematic region. Therefore, this work shows that the application of exogenous NO 2-OA inhibiting mitosis process further reduces the growth of the primary root.
Intracellular Ca2 + plays an important role in plant cell sensing various environmental stress signals by modulating the activity of Ca2 + -binding proteins. Leymus chinensis is the dominant forage grass widely distributed in the Eurasian Steppe which is well adapted to drought and salty soil common in the region. Through the transcript profile of the roots of L. chinensis, we identify the transcript is predicted to encode calcium-binding histidine-rich protein (HRC), the protein was recently marked in the grain.
L. chinensis HRC (LCH RC) locally in the core, as demonstrated using transient gene expression method that we developed for this species. LcHRC different regions show good affinity for Ca2 + or Zn 2+, but not Mg 2+ and Mn 2+. Arabidopsis thaliana seeds expressing heterologously LcHRC show greater sensitivity to abscisic acid (ABA), along with a decrease in the expression of several genes ABA-inducible markers, but no increase in ABA content.
Arabidopsis cDNA library screening to identify yeast Tudor / PWWP / MBT-domain-containing protein (AtPWWP3) which interacts with LcHRC. AtPWWP3 also localized in the nucleus and are thought to mediate gene expression by modifying histone deacetylation. Based on these results, we propose a functional model LcHRC action.
Exogenous Nitro-Oleic Acid Treatment Inhibits Primary Root Growth by Reducing the Mitosis in the Meristem in Arabidopsis thaliana
induced cell death in Arabidopsis hydrolase fumarylacetoacetate loss associated with the phytohormone jasmonate but not salicylic acid
Fumarylacetoacetate hydrolase (FAH) catalyzes the last step in the degradation of Tyr important pathway for animals but not well understood in plants. Previously, we found that mutations encoding the Arabidopsis FAH SSCD1 cause cell death under short days, discovered the important role of Tyr degradation pathways in plants.
Since phytohormones salicylic acid (SA) and jasmonate (JA) is involved in programmed cell death, in this study, we investigated whether cell death sscd1 associated with the SA and JA, and found that (1) accompanied by up-regulation of genes Janine and SA -induced well as the accumulation of JA but not SA; (2) it is pressed by splitting the signal but not SA JA signaling; (3) up-regulation of reactive oxygen species marker gene in sscd1 pressed by solving JA signaling; (4) the treatment of wild-type Arabidopsis with succinylacetone, abnormal metabolites resulting from loss of FAH, induces the expression of JA-induced genes, while treatment with JA induces expression of several genes
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.
Description: A polyclonal antibody for HSC70 from Plant | Wheat (Triticum spp.) | Pea (Pisum sativum). The antibody is produced in rabbit after immunization with Plant HSC70 purified from wheat germ. The Antibody is tested and validated for WB, ELISA, IP assays with the following recommended dilutions: WB (1:50000). This HSC70 antibody is unconjugated.
Description: A polyclonal antibody for HSP17.6 from Plant | Arabidopsis thaliana. The antibody is produced in rabbit after immunization with Plant Recombinant protein Arabidopsis thaliana Hsp17.6 CI (class one) . The Antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:1000). This HSP17.6 antibody is unconjugated.
Description: A polyclonal antibody for HSP17.7 from Plant | Arabidopsis thaliana. The antibody is produced in rabbit after immunization with Plant Full length recombinant protein HSP17.7 produced in E.coli and purified by conventional methods (no affinity tag). The Antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:1000). This HSP17.7 antibody is unconjugated.
Description: A polyclonal antibody for HSP21 from Plant | Arabidopsis thaliana. The antibody is produced in rabbit after immunization with Plant Recombinant Hsp21 protein derived from the sequence of Arabidopsis thaliana. The Antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:3000). This HSP21 antibody is unconjugated.
Description: A polyclonal antibody for HSP101 from Plant | Arabidopsis thaliana | Monocot (Agave tequilana). The antibody is produced in rabbit after immunization with Plant Recombinant protein Arabidopsis thaliana HSP101/ClpB N-terminal. The Antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:1000). This HSP101 antibody is unconjugated.
Description: A polyclonal antibody for HSP101 from Plant | Arabidopsis thaliana | Monocot (Agave tequilana). The antibody is produced in rabbit after immunization with Plant Recombinant protein Arabidopsis thaliana HSP101/ClpB C-terminal. The Antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:1000). This HSP101 antibody is unconjugated.
Description: A polyclonal antibody against Plant-actin. Recognizes Plant-actin from Arabidopsis Nicotiana tabacum Oryza sativa Hordeum vulgare. This antibody is Unconjugated. Tested in the following application: WB;WB:1:3000
Description: A polyclonal antibody against Plant GST. Recognizes Plant GST from Nicotiana tabacum. This antibody is Unconjugated. Tested in the following application: WB;WB:1:3000-10000
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Orange
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Potato
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Rice
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Plant Normal Tissue: cDNA from Plant: Wheat
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Plant Total RNA Mini Kit (50prep), for Woody Plant
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: This cell lysate is prepared from Rat Brain Tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from rat liver tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from rat lung tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from rat testis tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from rat kidney tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from rat spleen tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from rat thymus tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from rat heart tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from rat ovary tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from mouse brain tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from mouse liver tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from mouse testis tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from mouse ovary tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from mouse kidney tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from mouse spleen tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from mouse lung tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Tyr dependent degradation JA signaling. These results indicate that cell death in Arabidopsis FAH due to the loss associated with JA but not SA, and suggested that JA positive signals regulate cell death sscd1 by up-regulating the degradation of Tyr.
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 Control/blocking peptide)
 IgG, affinity pure)
 antiserum)
 IgG, affinity pure)
 IgG (aff pure))
 IgG affinity pure, loading control for WB)
 IgG, affinity pure)
 control/blocking peptide)
 IgG, affinity pure)
 IgG affinity pure, loading control for WB)
 IgG affinity pure, loading control for WB)
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 Antibody)
, for Woody Plant)
, for Woody Plant)
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-lysate Antibody)
 -lysate Antibody)
