Exogenous Nitro-Oleic Acid Treatment Inhibits Primary Root Growth by Reducing the Mitosis in the Meristem in Arabidopsis thaliana

August 20, 2020
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Nitric oxide (NO) is a second messenger that regulates various physiological processes in plants. NO-derived molecules called reactive nitrogen species (RNS) can react with unsaturated fatty acids produces fatty acids nitration (NO2-FA). NO2-FA work as a signaling molecule in mammals in which the production and the target has been described under different stress conditions. More recently, NO2-fatty acids were detected in plants, but their role (s) on plant physiological processes is still poorly known. Although in this work NO2-OA has not been detected in any tissue of Arabidopsis seedlings, here we show that the application of exogenous growth (NO2-OA) inhibits Arabidopsis primary root nitro-oleic acid;

This inhibition is not possible due to the production of oxide (NO) nitrate or disorder root auxin or cytokinin response. In-depth analysis shows that the roots were incubated with NO2-OA has a lower number of cells in the division. Although NO2-FA does not affect hormonal signaling mechanisms maintain the stem cell niche, the plants were incubated with NO2-OA showed a decrease in cell division in the meristematic region. Therefore, this work shows that the application of exogenous NO 2-OA inhibiting mitosis process further reduces the growth of the primary root.


Intracellular Ca2 + plays an important role in plant cell sensing various environmental stress signals by modulating the activity of Ca2 + -binding proteins. Leymus chinensis is the dominant forage grass widely distributed in the Eurasian Steppe which is well adapted to drought and salty soil common in the region. Through the transcript profile of the roots of L. chinensis, we identify the transcript is predicted to encode calcium-binding histidine-rich protein (HRC), the protein was recently marked in the grain.

L. chinensis HRC (LCH RC) locally in the core, as demonstrated using transient gene expression method that we developed for this species. LcHRC different regions show good affinity for Ca2 + or Zn 2+, but not Mg 2+ and Mn 2+. Arabidopsis thaliana seeds expressing heterologously LcHRC show greater sensitivity to abscisic acid (ABA), along with a decrease in the expression of several genes ABA-inducible markers, but no increase in ABA content.

Arabidopsis cDNA library screening to identify yeast Tudor / PWWP / MBT-domain-containing protein (AtPWWP3) which interacts with LcHRC. AtPWWP3 also localized in the nucleus and are thought to mediate gene expression by modifying histone deacetylation. Based on these results, we propose a functional model LcHRC action.

Exogenous Nitro-Oleic Acid Treatment Inhibits Primary Root Growth by Reducing the Mitosis in the Meristem in Arabidopsis thaliana
Exogenous Nitro-Oleic Acid Treatment Inhibits Primary Root Growth by Reducing the Mitosis in the Meristem in Arabidopsis thaliana

induced cell death in Arabidopsis hydrolase fumarylacetoacetate loss associated with the phytohormone jasmonate but not salicylic acid

Fumarylacetoacetate hydrolase (FAH) catalyzes the last step in the degradation of Tyr important pathway for animals but not well understood in plants. Previously, we found that mutations encoding the Arabidopsis FAH SSCD1 cause cell death under short days, discovered the important role of Tyr degradation pathways in plants.

Since phytohormones salicylic acid (SA) and jasmonate (JA) is involved in programmed cell death, in this study, we investigated whether cell death sscd1 associated with the SA and JA, and found that (1) accompanied by up-regulation of genes Janine and SA -induced well as the accumulation of JA but not SA; (2) it is pressed by splitting the signal but not SA JA signaling; (3) up-regulation of reactive oxygen species marker gene in sscd1 pressed by solving JA signaling; (4) the treatment of wild-type Arabidopsis with succinylacetone, abnormal metabolites resulting from loss of FAH, induces the expression of JA-induced genes, while treatment with JA induces expression of several genes

Plant Arabidopsis thaliana Cytochrome P450 83B1 (CYP83B1) ELISA Kit
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Plant Arabidopsis thaliana Cytochrome P450 83B1 (CYP83B1) ELISA Kit
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Recombinant Arabidopsis thaliana Novel plant SNARE 12 (NPSN12), partial
MBS1318719-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1120

Recombinant Arabidopsis thaliana Novel plant SNARE 12 (NPSN12), partial
MBS1318719-005mgMammalianCell 0.05mg(Mammalian-Cell)
EUR 1340

Recombinant Arabidopsis thaliana Novel plant SNARE 12 (NPSN12), partial
MBS1318719-05mgEColi 0.5mg(E-Coli)
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Recombinant Arabidopsis thaliana Novel plant SNARE 12 (NPSN12), partial
MBS1318719-05mgYeast 0.5mg(Yeast)
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Recombinant Arabidopsis thaliana Novel plant SNARE 12 (NPSN12), partial
MBS1318719-1mgEColi 1mg(E-Coli)
EUR 1645

Recombinant Arabidopsis thaliana Novel plant SNARE 13 (NPSN13), partial
MBS1453225-005mgBaculovirus 0.05mg(Baculovirus)
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Recombinant Arabidopsis thaliana Novel plant SNARE 13 (NPSN13), partial
MBS1453225-005mgMammalianCell 0.05mg(Mammalian-Cell)
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Recombinant Arabidopsis thaliana Novel plant SNARE 13 (NPSN13), partial
MBS1453225-05mgEColi 0.5mg(E-Coli)
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Recombinant Arabidopsis thaliana Novel plant SNARE 13 (NPSN13), partial
MBS1453225-05mgYeast 0.5mg(Yeast)
EUR 1340

Recombinant Arabidopsis thaliana Novel plant SNARE 13 (NPSN13), partial
MBS1453225-1mgEColi 1mg(E-Coli)
EUR 1645

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MBS1467108-005mgBaculovirus 0.05mg(Baculovirus)
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Recombinant Arabidopsis thaliana Novel plant SNARE 11 (NPSN11), partial
MBS1467108-005mgMammalianCell 0.05mg(Mammalian-Cell)
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Recombinant Arabidopsis thaliana Novel plant SNARE 11 (NPSN11), partial
MBS1467108-05mgEColi 0.5mg(E-Coli)
EUR 1120

Recombinant Arabidopsis thaliana Novel plant SNARE 11 (NPSN11), partial
MBS1467108-05mgYeast 0.5mg(Yeast)
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Recombinant Arabidopsis thaliana Novel plant SNARE 11 (NPSN11), partial
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MBS7025416-5x01mg 5x0.1mg
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Recombinant Arabidopsis thaliana Protein PLANT CADMIUM RESISTANCE 2 (PCR2)
MBS7025420-002mg 0.02mg
EUR 1425

Recombinant Arabidopsis thaliana Protein PLANT CADMIUM RESISTANCE 2 (PCR2)
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Recombinant Arabidopsis thaliana Protein PLANT CADMIUM RESISTANCE 2 (PCR2)
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Recombinant Arabidopsis thaliana Protein PLANT CADMIUM RESISTANCE 3 (PCR3)
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Recombinant Arabidopsis thaliana Protein PLANT CADMIUM RESISTANCE 3 (PCR3)
MBS7025421-01mg 0.1mg
EUR 2390

Recombinant Arabidopsis thaliana Protein PLANT CADMIUM RESISTANCE 3 (PCR3)
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EUR 10715

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MBS7025427-01mg 0.1mg
EUR 2385

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MBS7025427-5x01mg 5x0.1mg
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MBS7025419-5x01mg 5x0.1mg
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Plant-Derived Arabidopsis Lysate
IPLTARATL100UG each
EUR 541
Description: Plant-Derived Arabidopsis Lysate

Plant-Derived Arabidopsis Lysate
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Arabidopsis thaliana Arabidopsis thaliana (AUR1)
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Recombinant Arabidopsis thaliana Arabidopsis thaliana(AUR1)
AP74628 1mg
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Recombinant Arabidopsis thaliana Arabidopsis thaliana (AUR1)
MBS9424208-002mg 0.02mg
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Recombinant Arabidopsis thaliana Arabidopsis thaliana (AUR1)
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EUR 795

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MBS9424208-1mg 1mg
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Arabidopsis thaliana Protein ROS1 (ROS1)
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Description: Recombinant Arabidopsis thaliana Protein ROS1(ROS1),partial expressed in E.coli

Arabidopsis thaliana riboPOOL (6 reactions)
SIdp-K006-000008 each
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Arabidopsis thaliana riboPOOL (12 reactions)
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Recombinant Arabidopsis thaliana Remorin (DBP)
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EUR 1110

Recombinant Arabidopsis thaliana Remorin (DBP)
MBS1004094-002mgEColi 0.02mg(E-Coli)
EUR 705

Recombinant Arabidopsis thaliana Remorin (DBP)
MBS1004094-002mgYeast 0.02mg(Yeast)
EUR 860

Recombinant Arabidopsis thaliana Remorin (DBP)
MBS1004094-01mgEColi 0.1mg(E-Coli)
EUR 825

Recombinant Arabidopsis thaliana Remorin (DBP)
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Arabidopsis thaliana Dehydrin Xero 1 (XERO1)
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Description: Recombinant Arabidopsis thaliana Dehydrin Xero 1(XERO1) expressed in E.coli

PBA1 (Arabidopsis thaliana) polyclonal antibody
MBS566514-0025mL 0.025mL
EUR 275

PBA1 (Arabidopsis thaliana) polyclonal antibody
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Recombinant Arabidopsis thaliana Protein FD (FD)
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EUR 1165

Recombinant Arabidopsis thaliana Protein FD (FD)
MBS1295554-002mgEColi 0.02mg(E-Coli)
EUR 805

Recombinant Arabidopsis thaliana Protein FD (FD)
MBS1295554-002mgYeast 0.02mg(Yeast)
EUR 955

Recombinant Arabidopsis thaliana Protein FD (FD)
MBS1295554-01mgEColi 0.1mg(E-Coli)
EUR 965

Recombinant Arabidopsis thaliana Protein FD (FD)
MBS1295554-01mgYeast 0.1mg(Yeast)
EUR 1120

Recombinant Arabidopsis thaliana Actin-1 (ACT1)
MBS1078636-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1250

Recombinant Arabidopsis thaliana Actin-1 (ACT1)
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EUR 915

Recombinant Arabidopsis thaliana Actin-1 (ACT1)
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EUR 1045

Recombinant Arabidopsis thaliana Actin-1 (ACT1)
MBS1078636-01mgEColi 0.1mg(E-Coli)
EUR 1100

Recombinant Arabidopsis thaliana Actin-1 (ACT1)
MBS1078636-01mgYeast 0.1mg(Yeast)
EUR 1185

Recombinant Arabidopsis thaliana Actin-4 (ACT4)
MBS1218921-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1250

Recombinant Arabidopsis thaliana Actin-4 (ACT4)
MBS1218921-002mgEColi 0.02mg(E-Coli)
EUR 915

Recombinant Arabidopsis thaliana Actin-4 (ACT4)
MBS1218921-002mgYeast 0.02mg(Yeast)
EUR 1045

Recombinant Arabidopsis thaliana Actin-4 (ACT4)
MBS1218921-01mgEColi 0.1mg(E-Coli)
EUR 1100

Recombinant Arabidopsis thaliana Actin-4 (ACT4)
MBS1218921-01mgYeast 0.1mg(Yeast)
EUR 1185

Recombinant Arabidopsis thaliana Actin-7 (ACT7)
MBS1107414-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1250

Recombinant Arabidopsis thaliana Actin-7 (ACT7)
MBS1107414-002mgEColi 0.02mg(E-Coli)
EUR 915

Recombinant Arabidopsis thaliana Actin-7 (ACT7)
MBS1107414-002mgYeast 0.02mg(Yeast)
EUR 1045

Recombinant Arabidopsis thaliana Actin-7 (ACT7)
MBS1107414-01mgEColi 0.1mg(E-Coli)
EUR 1100

Recombinant Arabidopsis thaliana Actin-7 (ACT7)
MBS1107414-01mgYeast 0.1mg(Yeast)
EUR 1185

Recombinant Arabidopsis thaliana Actin-2 (ACT2)
MBS1299077-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1250

Recombinant Arabidopsis thaliana Actin-2 (ACT2)
MBS1299077-002mgEColi 0.02mg(E-Coli)
EUR 915

Recombinant Arabidopsis thaliana Actin-2 (ACT2)
MBS1299077-002mgYeast 0.02mg(Yeast)
EUR 1045

Recombinant Arabidopsis thaliana Actin-2 (ACT2)
MBS1299077-01mgEColi 0.1mg(E-Coli)
EUR 1100

Recombinant Arabidopsis thaliana Actin-2 (ACT2)
MBS1299077-01mgYeast 0.1mg(Yeast)
EUR 1185

Recombinant Arabidopsis thaliana Actin-3 (ACT3)
MBS1008184-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1250

Recombinant Arabidopsis thaliana Actin-3 (ACT3)
MBS1008184-002mgEColi 0.02mg(E-Coli)
EUR 915

Recombinant Arabidopsis thaliana Actin-3 (ACT3)
MBS1008184-002mgYeast 0.02mg(Yeast)
EUR 1045

Recombinant Arabidopsis thaliana Actin-3 (ACT3)
MBS1008184-01mgEColi 0.1mg(E-Coli)
EUR 1100

Recombinant Arabidopsis thaliana Actin-3 (ACT3)
MBS1008184-01mgYeast 0.1mg(Yeast)
EUR 1185

Recombinant Arabidopsis thaliana Pirin-1 (PRN1)
MBS1470913-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1165

Recombinant Arabidopsis thaliana Pirin-1 (PRN1)
MBS1470913-002mgEColi 0.02mg(E-Coli)
EUR 805

Recombinant Arabidopsis thaliana Pirin-1 (PRN1)
MBS1470913-002mgYeast 0.02mg(Yeast)
EUR 955

Recombinant Arabidopsis thaliana Pirin-1 (PRN1)
MBS1470913-01mgEColi 0.1mg(E-Coli)
EUR 970

Recombinant Arabidopsis thaliana Pirin-1 (PRN1)
MBS1470913-01mgYeast 0.1mg(Yeast)
EUR 1120

Recombinant Arabidopsis thaliana Actin-8 (ACT8)
MBS1373395-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1250

Recombinant Arabidopsis thaliana Actin-8 (ACT8)
MBS1373395-002mgEColi 0.02mg(E-Coli)
EUR 915

Recombinant Arabidopsis thaliana Actin-8 (ACT8)
MBS1373395-002mgYeast 0.02mg(Yeast)
EUR 1045

Recombinant Arabidopsis thaliana Actin-8 (ACT8)
MBS1373395-01mgEColi 0.1mg(E-Coli)
EUR 1100

Recombinant Arabidopsis thaliana Actin-8 (ACT8)
MBS1373395-01mgYeast 0.1mg(Yeast)
EUR 1185

Arabidopsis thaliana Cyclin-B1-1 (CYCB1-1)
1-CSB-EP339075DOA
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Description: Recombinant Arabidopsis thaliana Cyclin-B1-1(CYCB1-1) expressed in E.coli

Arabidopsis thaliana Cyclin-B2-2 (CYCB2-2)
1-CSB-YP654394DOA
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Description: Recombinant Arabidopsis thaliana Cyclin-B2-2(CYCB2-2) expressed in Yeast

Recombinant Arabidopsis thaliana Histone H3.2 (HTR2)
MBS1164276-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1055

Recombinant Arabidopsis thaliana Histone H3.2 (HTR2)
MBS1164276-002mgEColi 0.02mg(E-Coli)
EUR 640

Recombinant Arabidopsis thaliana Histone H3.2 (HTR2)
MBS1164276-002mgYeast 0.02mg(Yeast)
EUR 810

Recombinant Arabidopsis thaliana Histone H3.2 (HTR2)
MBS1164276-01mgEColi 0.1mg(E-Coli)
EUR 745

Recombinant Arabidopsis thaliana Histone H3.2 (HTR2)
MBS1164276-01mgYeast 0.1mg(Yeast)
EUR 950

Recombinant Arabidopsis thaliana Histone H3.3 (HTR4)
MBS1189830-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1055

Recombinant Arabidopsis thaliana Histone H3.3 (HTR4)
MBS1189830-002mgEColi 0.02mg(E-Coli)
EUR 640

Recombinant Arabidopsis thaliana Histone H3.3 (HTR4)
MBS1189830-002mgYeast 0.02mg(Yeast)
EUR 810

Recombinant Arabidopsis thaliana Histone H3.3 (HTR4)
MBS1189830-01mgEColi 0.1mg(E-Coli)
EUR 745

Recombinant Arabidopsis thaliana Histone H3.3 (HTR4)
MBS1189830-01mgYeast 0.1mg(Yeast)
EUR 950

Recombinant Arabidopsis thaliana Amidase 1 (AMI1)
MBS1313461-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1295

Recombinant Arabidopsis thaliana Amidase 1 (AMI1)
MBS1313461-002mgEColi 0.02mg(E-Coli)
EUR 975

Recombinant Arabidopsis thaliana Amidase 1 (AMI1)
MBS1313461-002mgYeast 0.02mg(Yeast)
EUR 1090

Recombinant Arabidopsis thaliana Amidase 1 (AMI1)
MBS1313461-01mgEColi 0.1mg(E-Coli)
EUR 1140

Recombinant Arabidopsis thaliana Amidase 1 (AMI1)
MBS1313461-01mgYeast 0.1mg(Yeast)
EUR 1240

Recombinant Arabidopsis thaliana Protein IDA (IDA)
MBS1288868-002mgBaculovirus 0.02mg(Baculovirus)
EUR 975

Recombinant Arabidopsis thaliana Protein IDA (IDA)
MBS1288868-002mgEColi 0.02mg(E-Coli)
EUR 540

Recombinant Arabidopsis thaliana Protein IDA (IDA)
MBS1288868-002mgYeast 0.02mg(Yeast)
EUR 730

Recombinant Arabidopsis thaliana Protein IDA (IDA)
MBS1288868-01mgEColi 0.1mg(E-Coli)
EUR 635

Recombinant Arabidopsis thaliana Protein IDA (IDA)
MBS1288868-01mgYeast 0.1mg(Yeast)
EUR 855

Recombinant Arabidopsis thaliana Annexin D5 (ANN5)
MBS1317419-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1195

Recombinant Arabidopsis thaliana Annexin D5 (ANN5)
MBS1317419-002mgEColi 0.02mg(E-Coli)
EUR 845

Recombinant Arabidopsis thaliana Annexin D5 (ANN5)
MBS1317419-002mgYeast 0.02mg(Yeast)
EUR 985

Recombinant Arabidopsis thaliana Annexin D5 (ANN5)
MBS1317419-01mgEColi 0.1mg(E-Coli)
EUR 1015

Recombinant Arabidopsis thaliana Annexin D5 (ANN5)
MBS1317419-01mgYeast 0.1mg(Yeast)
EUR 1120

Recombinant Arabidopsis thaliana Annexin D6 (ANN6)
MBS1486347-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1195

Tyr dependent degradation JA signaling. These results indicate that cell death in Arabidopsis FAH due to the loss associated with JA but not SA, and suggested that JA positive signals regulate cell death sscd1 by up-regulating the degradation of Tyr.

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